A reactive oxygen species burst causes haploid induction in maize

文献类型： 外文期刊

第一作者： Jiang, Chenglin

作者： Jiang, Chenglin;Sun, Ju;Li, Rui;Chen, Wei;Guo, Liang;Luo, Cheng;Zhang, Qinghua;Jackson, David;Yan, Jianbing;Yan, Shijuan;Huang, Wenjie;Qin, Guochen;Wang, Pengcheng;Fernie, Alisdair R.;Jackson, David;Li, Xiang;Li, Xiang;Yan, Jianbing

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关键词： haploid induction; single-cell sequencing; maize; ROS; peroxidase

期刊名称：MOLECULAR PLANT （ 影响因子：21.949； 五年影响因子：19.617 ）

ISSN： 1674-2052

年卷期： 2022 年 15 卷 6 期

页码：

收录情况： SCI

摘要： Haploid induction (HI) is an important tool in crop breeding. Phospholipase A1 (ZmPLA1)/NOT LIKE DAD (NLD)/MATRILINEAL (MTL) is a key gene controlling HI in maize; however, the underlying molecular mech-anism remains unclear. In this study, to dissect why loss of ZmPLA1 function could mediate HI we per -formed a comprehensive multiple omics analysis of zmpla1 mutant anthers by integrating transcriptome, metabolome, quantitative proteome, and protein modification data. Functional classes of significantly en-riched or differentially abundant molecular entities were found to be associated with the oxidative stress response, suggesting that a reactive oxygen species (ROS) burst plays a critical role in HI. In support of this, we further discovered that a simple chemical treatment of pollen with ROS reagents could lead to HI. Moreover, we identified ZmPOD65, which encodes a sperm-specific peroxidase, as a new gene control-ling HI. Taken together, our study revealed a likely mechanism of HI, discovered a new gene controlling HI, and created a new method for HI in maize, indicating the importance of ROS balance in maintaining normal reproduction and providing a potential route to accelerate crop breeding.

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