Mapping the self-interacting domains of TuMV HC-Pro and the subcellular localization of the protein

文献类型： 外文期刊

第一作者： Zheng, Hongying

作者： Zheng, Hongying;Yan, Fei;Lu, Yuwen;Sun, Liying;Lin, Lin;Chen, Jianping;Zheng, Hongying;Cai, Li;Hou, Mingsheng

作者机构：

关键词： Turnip mosaic virus;HC-Pro;Subcellular localization;Interaction;YTH;BiFC

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN： 0920-8569

年卷期： 2011 年 42 卷 1 期

页码：

收录情况： SCI

摘要： The helper component-proteinase (HC-Pro) of potyviruses is a multifunctional protein involved in aphid transmission, polyprotein processing, cell-to-cell and long-distance movement, genome amplification and symptom expression. The HC-Pros of several potyviruses interact with themselves but the key domains responsible for self-interaction are apparently not conserved. In our experiments, yeast two-hybrid assays and bimolecular fluorescence complementation showed that Turnip mosaic virus (TuMV) HC-Pro interacted with itself in yeast cells, plant cells and insect cells. It was also shown that the central and C-terminal regions of the HC-Pro participated in these self-interactions. Fluorescence microscopy showed that TuMV HC-Pro was present in the cytoplasm and formed aggregates along the ER.

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