Development and polymorphism of Vigna unguiculata ssp unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp sesquipedialis (L.) Verdc.)

文献类型： 外文期刊

第一作者： Xu, Pei

作者： Xu, Pei;Wu, Xiaohua;Wang, Baogen;Liu, Yonghua;Hu, Tingting;Lu, Zhongfu;Li, Guojing;Qin, Dehui;Ehlers, Jeffery D.;Close, Timothy J.

作者机构：

关键词： Asparagus bean;Cowpea;Genetic diversity;RIL;SSR;Vigna

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2010 年 25 卷 4 期

页码：

收录情况： SCI

摘要： Asparagus bean (V. unguiculata ssp. sesquipedialis), a specific form of cowpea (V. unguiculata L. Walp.), is cultivated as a vegetable crop throughout eastern and southern Asia for its tender long pods. Little is known about the genetic relationship between asparagus bean and the broader species, particularly the dominant ssp. unguiculata. We report here the development and transferability of simple sequence repeat (SSR) markers, over 40% of which are EST-derived, from ssp. unguiculata to asparagus bean and the use of a subset of the polymorphic markers to assess the genetic diversity of asparagus bean cultivars from diverse geographic origins across China. A total of 410 EST derived SSR (eSSR) markers and 600 SSR markers derived from cowpea genespace sequences (GSS) were developed, with a cross-subspecies transferability of 100% and 98.5%, respectively. In a recombinant inbred line population of asparagus bean, a 1:1 segregation was observed for most loci. Principal coordinate analysis (PCA) and phylogenetic clustering based on 62 alleles detected by 14 polymorphic SSR markers distinguished ssp. unguiculata and sesquipedialis into separate groups. Improved asparagus bean cultivars in China generally have a narrow genetic basis compared with landraces varieties. This suggests that asparagus bean breeding programs need to consider utilizing landrace germplasm to enhance genetic variability and ensure long-term gains from selection and reduce genetic vulnerability to pathogen/pest epidemics. Because of their transferability across subspecies, the SSR markers described in this study could be effectively employed in cross-subspecies trait introgression breeding from ssp. unguiculata to sesquipedialis.

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