Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction

文献类型: 外文期刊

第一作者: Eusebio-Cope, Ana

作者: Eusebio-Cope, Ana;Suzuki, Nobuhiro;Sun, Liying;Hillman, Bradley I.

作者机构:

关键词: Mycovirus;Reovirus;Rearrangement;Virus transmission;Hypovirulence;dsRNA;Chestnut blight;Virocontrol;Cryphonectria parasitica;Symptom expression

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

ISSN: 0042-6822

年卷期: 2010 年 397 卷 2 期

页码:

收录情况: SCI

摘要: Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4. albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 Plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent. (C) 2009 Elsevier Inc. All rights reserved.

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