Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers

文献类型： 外文期刊

第一作者： Xiao JinPing

作者： Xiao JinPing;Chen LiGeng;Liu HaiLin;Xie Ming;Ye WeiQi

作者机构：

关键词： Seedlessness;AFLP;Ponkan mandarin;SCAR

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2009 年 121 卷 4 期

页码：

收录情况： SCI

摘要： Seedlessness is a desirable trait ill Citrus and has been an important breeding objective. In this Study, we employed the amplified fragment length polymorphism (AFLP) technique to find molecular markers linked to the seedless trait in the Ponkan mandarin (Citrus reticulata Blanco). After screening with 72 primer pairs, 5 AFLP markets were identified that putatively correlated with the target trait. Their association was also tested by analyzing the AFLP profile from pooled individual accessions. The five fragments were cloned and sequenced, and BLAST searches showed that four of the markers had high homology to functional genes, providing some promising information that may aid in understanding the molecular mechanism of seedlessness in citrus. Based on the sequence information, eight specific primers were designed and eventually fragments AFLP-2 and AFLP-5 were successfully converted into sequence characterized amplified region (SCAR) markers. Thus, the markers detected could be useful for accelerating citrus breeding programmes by enabling early screening for seedlessness mutations using marker-assisted selection. (c) 2009 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Development of molecular markers linked to the traits of non-acid/acid fruit of peach. Wu, Jun,Shu, Huairui,Zhang, Kaichun,Jiang, Lijie. 2007

[2]Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa). Wang, Y.,Bi, B.,Yuan, Q. H.,Li, X. L.,Gao, J. M.. 2012

[3]Isolation of Female-Specific AFLP Markers and Molecular Identification of Genetic Sex in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Li, Jing,Deng, Si-Ping,Tian, Yong-Sheng,Wang, Qing-Yin,Zhuang, Zhi-Meng,Sha, Zhen-Xia,Xu, Jian-Yong.

[4]Identification of male-specific AFLP and SCAR markers in the dioecious plant Humulus scandens. Li, Shu-Fen,Deng, Chuan-Liang,Gao, Wu-Jun,Wang, Lian-Jun.

[5]Digital Gene Expression Analysis of Ponkan Mandarin (Citrus reticulata Blanco) in Response to Asia Citrus Psyllid-Vectored Huanglongbing Infection. Zhong, Yun,Cheng, Chunzhen,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan,Cheng, Chunzhen,Zhang, Yongyan,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan. 2016

[6]Amplified fragment length polymorphism analysis of Mythimna separata (Lepidoptera : Noctuidae) geographic and melanie laboratory populations in China. Luo, Li-Zhi,Zhang, Lei.

[7]Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp. I. In vitro embryo rescue and plant development. Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Niu, Liang. 2008

[8]Identification of favorable SNP alleles and candidate genes for seedlessness in Vitis vinifera L. using genome-wide association mapping. Zhang, Heng,Fan, Xiucai,Zhang, Ying,Jiang, Jianfu,Liu, Chonghuai.

[9]Several Low-Seeded Citrus Hybrids Selected from a Tangor x 'Ponkan' Cross. Chen, Ke-Ling,He, Jian,Guan, Bin.

[10]Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Larkin, PJ. 2001

[11]A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.). Wang, L. H.,Gu, X. H.,Hua, M. Y.,Mao, S. L.,Zhang, Z. H.,Zhang, B. X.,Gu, X. H.,Hua, M. Y.,Yun, X. F.,Peng, D. L..

[12]Identification of molecular markers for resistance to Septoria nodorum blotch in durum wheat. Cao, W,Hughes, GR,Ma, H,Dong, Z. 2001

[13]Mitochondrial SCAR and SSR Markers for distinguishing cytoplasmic male sterile lines from their isogenic maintainer lines in cotton. Zhang, Xiao,Meng, Zhigang,Zhou, Tao,Sun, Guoqing,Shi, Ji,Zhang, Rui,Guo, Sandui,Zhang, Xiao,Yu, Yuanhua.

[14]Morphological, cytological and molecular characterization of a novel symmetric somatic hybrid between N-tabacum and N-glauca. Sun, Y.,Xue, Q.,Zhang, X.,Ding, C.,Zhang, L.,Gong, M.,Bu, G.,Wang, W.. 2007

[15]Different reactions to the wheat curl mite and Wheat streak mosaic virus in various wheat-Haynaldia villosa 6V and 6VS lines. Li, HJ,Conner, RL,Chen, Q,Jia, X,Li, H,Graf, RJ,Laroche, A,Kuzyk, AD. 2002

[16]Development of PCR-based markers linked to a restorer gene for cytoplasmic male sterility in radish (Raphanus sativus L.). Wang, Zhiwei,Xiang, Changping,Mei, Shiyong. 2006

[17]Autopolyploidy leads to rapid genomic changes in Arabidopsis thaliana. Liu, Shihong,Tian, Baoming,Wei, Fang.

[18]An assessment of the genetic diversity within Ganoderma strains with AFLP and ITS PCR-RFLP. Yang, Zhirong,Zheng, Linyong,Jia, Dinghong,Fei, Xiaofan,Luo, Xia. 2009

[19]IDENTIFICATION OF EXOTIC GENETIC COMPONENTS AND DNA METHYLATION PATTERN ANALYSIS OF THREE COTTON INTROGRESSION LINES FROM Gossypium bickii. Shou Pu He,Jun Ling Sun,Chao Zhang,Xiong Ming Du.

[20]Genetic and gene expression analysis of dm1, a dwarf mutant from Cucurbita maxima Duch. ex Lam, based on the AFLP method. Wang, Rui,Huang, Hexun,Lin, Yu'e,Liang, Zhaojun,Wu, Tingquan,Wang, Rui,Wu, Tingquan,Chen, Qinghua. 2014

作者其他论文 更多>>

微信小程序