Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers

文献类型： 外文期刊

第一作者： Xiao JinPing

作者： Xiao JinPing;Chen LiGeng;Liu HaiLin;Xie Ming;Ye WeiQi

作者机构： Zhejiang Univ, Lab Fruit Mol Physiol & Biotechnol, State Agr Minist Lab Hort Plant Growth Dev & Biot, Hangzhou 310029, Zhejiang, Peoples R China;Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China;Lishui Inst Agr Sci, Lishui 323000, Peoples R China

关键词： Seedlessness;AFLP;Ponkan mandarin;SCAR

期刊名称：SCIENTIA HORTICULTURAE （ 2020影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2009 年 121 卷 4 期

页码：

收录情况： SCI

摘要： Seedlessness is a desirable trait ill Citrus and has been an important breeding objective. In this Study, we employed the amplified fragment length polymorphism (AFLP) technique to find molecular markers linked to the seedless trait in the Ponkan mandarin (Citrus reticulata Blanco). After screening with 72 primer pairs, 5 AFLP markets were identified that putatively correlated with the target trait. Their association was also tested by analyzing the AFLP profile from pooled individual accessions. The five fragments were cloned and sequenced, and BLAST searches showed that four of the markers had high homology to functional genes, providing some promising information that may aid in understanding the molecular mechanism of seedlessness in citrus. Based on the sequence information, eight specific primers were designed and eventually fragments AFLP-2 and AFLP-5 were successfully converted into sequence characterized amplified region (SCAR) markers. Thus, the markers detected could be useful for accelerating citrus breeding programmes by enabling early screening for seedlessness mutations using marker-assisted selection. (c) 2009 Elsevier B.V. All rights reserved.

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