Cloning, sequencing and expression of a novel xylanase cDNA from a newly isolated Aspergillus awamori in Pichia pastoris

文献类型： 外文期刊

第一作者： Ping, Lifeng

作者： Ping, Lifeng;Yang, Yinan;Lu, Jingmei;Yang, Yinan;Shan, Kuixian

作者机构：

关键词： Aspergillus awamori;cDNA;cloning;xylanase;eukaryotic expression;purification and characterization

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2008 年 7 卷 23 期

页码：

收录情况： SCI

摘要： A strain SH 2016, capable of producing xylanase, was isolated and identified as Aspergillus awamori, based on its physiological and biochemical characteristics as well as its ITS rDNA gene sequence analysis. A xylanase gene of 591 bp was cloned from this newly isolated A. awamori and the ORF sequence predicted a protein of 196 amino acids with a molecular mass about 21 kDa. An expression plasmid carrying the gene under the control of the methanol regulated alcohol oxidase gene (AOX1) promotor was introduced into Pichia pastoris, and xylanase gene was successfully expressed into the medium using methanol as inducer. Xylanase with 6 his tags was purified using Ni2+-NTA column. The characteristics of purified xylanase were investigated.

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