Proteome Analysis on Differentially Expressed Proteins of the Fat Body of Two Silkworm Breeds, Bombyx mori, Exposed to Heat Shock Exposure

文献类型： 外文期刊

第一作者： Moghaddam, S. Hossein Hosseini

作者： Moghaddam, S. Hossein Hosseini;Du, Xin;Li, Jun;Zhong, Boxiong;Chen, YuYin;Moghaddam, S. Hossein Hosseini;Cao, Jinru

作者机构：

关键词： proteome analysis;heat shock proteins;silkworm;2D electrophoresis;mass spectrometry

期刊名称：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING （ 影响因子：2.836； 五年影响因子：2.281 ）

ISSN： 1226-8372

年卷期： 2008 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Proteomes of heat tolerant (multivoltine) and heat susceptible (bivoltine) silkworms (Bombyx mori) in response to heat shock were studied. Detected proteins from fat body were identified by using MALDI-TOF/TOF spectrometer, MS/MS, and MS analysis. Eight proteins, including small heat shock proteins (sHSPs) and HSP70, were expressed similarly in both breeds, while 4 protein spots were expressed specifically in the bivoltine breed and 12 protein spots were expressed specifically in the multivoltine breed. In the present proteomics approach, 5 separate spots of sHSP proteins (HSP19.9, HSP20.1, HSP20.4, HSP20.8, and HSP21.4) were identified. Protein spot intensity of sHSPs was lower in the multivoltine breed than in the bivoltine breed after the 45 degrees C heat shock treatment, while the difference between two breeds was not significant after the 41 degrees C heat shock treatment. These results indicated that some other mechanisms might be engaged in thermal tolerance of multivotine breed except for the expression of sHSP and HSP70. There were visible differences in the intensity of heat shock protein expression between male and female, however, differences were not statistically significant. (C) KSBB

分类号：

  • 相关文献

[1]Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics. Shen, Weifeng,Shen, Weifeng,Meng, Zhiqi,Han, Wei,Li, Yunong,Li, Liang,Cai, Leiming.

[2]Cloning and expression of heat shock protein genes in two whitefly species in response to thermal stress. Wan, F. -H.,Yu, H.,Wan, F. -H..

[3]Genome shuffling of Propionibacterium shermanii for improving vitamin B12 production and comparative proteome analysis. Zhang, Ying,Liu, Jian-Zhong,Mao, Zong-Wan,Zhang, Ying,Liu, Jian-Zhong,Mao, Zong-Wan,Huang, Jun-Sheng. 2010

[4]Protein Expression Changes in Human Monocytic THP-1 Cells Treated with Lipoteichoic Acid from Lactobacillus plantarum and Staphylococcus aureus. Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Lee, Hae Young,Jung, Bong Jun,Chung, Dae Kyun,Zeng, Ri-Zhong,Kim, Han Geun,Kim, Na Ra,Lee, Hae Young,Jung, Bong Jun,Chung, Dae Kyun,Zeng, Ri-Zhong,Ko, Mi Yeon,Lee, Seung Yeon,Chung, Dae Kyun,Chung, Dae Kyun. 2010

[5]Proteomics analysis reveals distinct involvement of embryo and endosperm proteins during seed germination in dormant and non-dormant rice seeds. Xu, Heng-Heng,Liu, Shu-Jun,Wang, Wei-Qing,Song, Song-Quan,Song, Shun-Hua,Wang, Rui-Xia.

[6]Overexpression of AtHsp90.3 in Arabidopsis thaliana impairs plant tolerance to heavy metal stress. Song, H. M.,Wang, H. Z.,Xu, X. B.,Song, H. M.. 2012

[7]Roles of heat-shock protein 70 in protecting against intestinal mucosal damage. Wu, Xin,Zhang, Yuzhe,Yin, Yulong,Wu, Xin,Yin, Yulong,Ruan, Zheng,Wu, Xin,Yin, Yulong,Ruan, Zheng,Yu, Huimin,Wu, Zhenlong,Wu, Guoyao,Wu, Guoyao. 2013

[8]Heat-induced apoptosis and gene expression in bovine mammary epithelial cells. Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan,Hu, Han,Wang, Jiaqi,Gao, Haina,Li, Songli,Zhang, Yangdong,Zheng, Nan.

[9]Molecular cloning, characterization, and heterologous expression analysis of heat shock protein genes (hsp70 and hsp90) of the invasive alien weed, Ageratina adenophora (Asteraceae). Wan, Fang Hao,Guo, Jian Ying,Gong, Wei Na,Xie, Bing Yan.

[10]Yeast heat-shock protein gene HSP26 enhances freezing tolerance in Arabidopsis. Xue, Yong,Peng, Rihe,Xiong, Aisheng,Li, Xian,Yao, Quanhong,Zha, Dingshi.

[11]CHIP mediates down-regulation of nucleobindin-1 in preosteoblast cell line models. Xue, Fuying,Zhao, Xinghui,Zhao, Taoran,Meng, Ying,Guo, Junwei,Chen, Wei,Wu, Yanping,Zhao, Zhanzhong.

[12]Effect of high dietary carbohydrate on growth, serum physiological response, and hepatic heat shock cognate protein 70 expression of the top-mouth culter Erythroculter ilishaeformis Bleeker. Liu, Bo,Xie, Jun,Ge, Xian-ping,Wang, Guangyu,Liu, Bo,Xie, Jun,Ge, Xian-ping,Miao, Ling-hong.

[13]Transient changes of enzyme activities and expression of stress proteins in the small intestine of piglets after weaning. Tao, Xin,Xu, Ziwei,Men, Xiaoming.

[14]Three heat shock proteins from Spodoptera exigua: Gene cloning, characterization and comparative stress response during heat and cold shocks. Xu, Qi,Zou, Qi,Zheng, Huizhen,Tang, Bin,Wang, Shigui,Zhang, Fan,Tang, Bin.

[15]Effects of heat stress on antioxidant defense system, inflammatory injury, and heat shock proteins of Muscovy and Pekin ducks: evidence for differential thermal sensitivities. Zeng, Tao,Wang, Gen-lin,Lu, Li-zhi,Zeng, Tao,Li, Jin-jun,Wang, De-qian,Li, Guo-qin,Lu, Li-zhi.

[16]RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Xing, Dongxu,Jiang, Liang,Xia, Qingyou,Xing, Dongxu,Yang, Qiong,Li, Qingrong,Xiao, Yang,Ye, Mingqiang. 2017

[17]Bombyx mori transcription factors: Genome-wide identification, expression profiles and response to pathogens by microarray analysis. Cheng, Tingcai,Xu, Pingzhen,Fang, Ting,Xia, Qingyou,Huang, Lulin. 2012

[18]Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution. Gui, Zhong Zheng,Kim, Bo Yeon,Lee, Kwang Sik,Wei, Ya Dong,Sohn, Hung Dae,Jin, Byung Rae,Gui, Zhong Zheng,Wei, Ya Dong,Guo, Xijie. 2008

[19]Mulberry nutrient management for silk production in Hubei Province of China. Chen, Fang,Wan, Kaiyuan,Lu, Jianwei,Zhang, Mingchu,Liu, Dongbi. 2009

[20]Transgenic silkworm of anti-NPV ribozyme. Zhang, F,Chen, X,Zhao, Y,Qi, GR,Huang, JT,Lu, CD. 1999

作者其他论文 更多>>

微信小程序