A perfect marker for fragrance genotyping in rice

文献类型： 外文期刊

第一作者： Bradbury, LMT

作者： Bradbury, LMT;Henry, RJ;Jin, QS;Reinke, RF;Waters, DLE

作者机构：

关键词： aromatic;assay;basmati;high-throughput;jasmine;Oryza sativa

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN： 1380-3743

年卷期： 2005 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Allele specific amplification (ASA) is a low-cost, robust technique that can be utilised to discriminate between alleles that differ by SNP's, insertions or deletions, within a single PCR tube. Fragrance in rice, a recessive trait, has been shown to be due to an eight bp deletion and three SNP's in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2). Here we report a single tube ASA assay which allows discrimination between fragrant and non-fragrant rice varieties and identifies homozygous fragrant, homozygous non-fragrant and heterozygous non-fragrant individuals in a population segregating for fragrance. External primers generate a fragment of approximately 580 bp as a positive control for each sample. Internal and corresponding external primers produce a 355 bp fragment from a non-fragrant allele and a 257 bp fragment from a fragrant allele, allowing simple analysis on agarose gels.

分类号：

  • 相关文献

[1]The gene for fragrance in rice. Bradbury, LMT,Fitzgerald, TL,Henry, RJ,Jin, QS,Waters, DLE.

[2]HEAT SHOCK INDUCED MORPHO-PHYSIOLOGICAL RESPONSE IN INDICA RICE (ORYZA SATIVA L.) AT EARLY SEEDLING STAGE. Zafar, Syed Adeel,Khan, Abdus Salam,Hameed, Amjad,Ashraf, Muhammad,Zafar, Syed Adeel. 2017

[3]Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb. Zhang, Qi,Wu, Yunru,Wang, Limin,Hu, Baishi,Liu, Fengquan,Zhang, Qi,Li, Peiwu.

[4]Development of an enzyme-linked immunosorbent assay for toosendanin. Zhang, Jing,Feng, Gang,Luo, Li,Ma, Zhi Qing,Feng, Jun Tao,Zhang, Xing,Yu, Xiang Yang,Liu, Xian Jin.

[5]Novel Cyclic Hexapeptides from Marine-Derived Fungus, Aspergillus sclerotiorum PT06-1. Zheng, Jinkai,Wang, Yi,Liu, Peipei,Wang, Xin,Peng, Xiaoping,Zhu, Weiming,Zhu, Huajie,Hong, Kui.

[6]Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application. Guo, Changying,Wu, Xia,Xu, Wei,Yang, Jinghe,Guo, Changying,Wu, Xia,Xu, Wei,Yang, Jinghe,Guo, Changying.

[7]A temperature-tolerant multiplex elements and genes screening system for genetically modified organisms based on dual priming oligonucleotide primers and capillary electrophoresis. Fu, Wei,Wang, Chenguang,Zhu, Pengyu,Zhu, Shuifang,Wei, Shuang,Wang, Chenguang,Du, Zhixin,Wu, Xiyang,Wu, Gang.

[8]Establishment of a Sensitive Nitric Oxide Bioassay for Bioscreening Application. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

[9]Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-throughput Microplate Format. Zhang, Xiaoling,Yang, Qiao. 2013

[10]High-throughput, low-cost, and event-specific polymerase chain reaction detection of herbicide tolerance in genetically modified soybean A2704-12. Ma, H.,Ma, H.,Li, H.,Li, J.,Wang, X. F.,Wei, P. C.,Li, L.,Yang, J. B.,Li, H.,Wei, P. C.,Li, H.,Li, J.. 2014

[11]Unmanned Aerial Vehicle Remote Sensing for Field-Based Crop Phenotyping: Current Status and Perspectives. Yang, Guijun,Liu, Jiangang,Zhao, Chunjiang,Yu, Haiyang,Xu, Bo,Yang, Xiaodong,Feng, Haikuan,Zhao, Xiaoqing,Li, Zhenhai,Li, Heli,Yang, Hao,Yang, Guijun,Zhao, Chunjiang,Yang, Xiaodong,Li, Zhenhai,Li, Heli,Yang, Hao,Yang, Guijun,Liu, Jiangang,Zhao, Chunjiang,Yu, Haiyang,Xu, Bo,Li, Zhenhong,Huang, Yanbo,Zhu, Dongmei,Zhang, Xiaoyan,Zhang, Ruyang. 2017

[12]Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids. Yang, Qiao,Zhang, Xiaoling,Huang, Hongliang. 2014

[13]SDR-ELISA: Ultrasensitive and high-throughput nucleic acid detection based on antibody-like DNA nanostructure. Wen, Junlin,Wen, Junlin,Chen, Junhua,Zhuang, Li,Zhou, Shungui,Wen, Junlin.

[14]Effects of elevated air temperature on physiological characteristics of flag leaves and grain yield in rice. Liu, Qi-Hua,Wu, Xiu,Ma, Jia-Qing,Zhou, Xue-Biao,Li, Tian. 2013

[15]Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines. Ding, Han-Feng,Liu, Xu,Li, Run-Fang,Wang, Wen-Ying,Zhang, Y.,Zhang, Xiao-Dong,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song,Ding, Han-Feng.

[16]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[17]Application of silicon fertilizer affects nutritional quality of rice. Liu, Qihua,Zhou, Xuebiao,Sun, Zhaowen. 2017

[18]Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions. Zhong, Zhengzheng,Zhang, Xin,Cheng, Zhijun,Wang, Jiulin,Wan, Jianmin,Zhong, Zhengzheng,Wu, Weixun,Wang, Hongjun,Chen, Liping,Liu, Linglong,Wang, Chunming,Zhao, Zhigang,Lu, Guangwen,Gao, He,Wei, Xiangjin,Yu, Chuanyuan,Chen, Mingjiang,Shen, Yingyue,Jiang, Ling,Wan, Jianmin. 2014

[19]Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences. Gao, Zhen-Yu,Guo, Long-Biao,Peng, You-Lin,Rao, Yu-Chun,Dong, Guo-Jun,Hu, Jiang,Liu, Hui-Juan,Xu, Jie,Zeng, Da-Li,Gong, Li-Hui,Zhang, Guang-Heng,Tian, Fu-Kuan,Xue, Da-Wei,Zhu, Li,Cheng, Shi-Hua,Qian, Qian,Zhao, Shan-Cen,He, Wei-Ming,Wang, Jin-Jin,Guo, Xiao-Sen,Zhang, Xue-Mei,Zhang, Chi,Zheng, Feng-Ya,Lu, Chang-Xin,Zhou, Qing,Wu, Hai-Yang,Ni, Pei-Xiang,Liu, Deng-Hui,Ye, Chen,Wang, Jian,Zhang, Geng-Yun,Wang, Jun,Zhang, Geng-Yun,Zhao, Shan-Cen,Zheng, Feng-Ya,Zhao, Shan-Cen,Zheng, Feng-Ya,Rao, Yu-Chun,Tian, Peng,Liao, Yi,Chen, Ming-Sheng,Li, Jia-Yang.

[20]The germin-like protein OsGLP2-1 enhances resistance to fungal blast and bacterial blight in rice. Liu, Qing,Zhang, Shaohong,Zhao, Junliang,Yang, Tifeng,Wang, Xiaofei,Mao, Xingxue,Dong, Jingfang,Liu, Bin,Liu, Qing,Zhang, Shaohong,Zhao, Junliang,Yang, Tifeng,Wang, Xiaofei,Mao, Xingxue,Dong, Jingfang,Liu, Bin,Yang, Jianyuan,Wang, Wenjuan,Zhu, Xiaoyuan,Yan, Shijuan.

作者其他论文 更多>>

微信小程序