Recombinant fusion protein and DNA vaccines against foot and mouth disease virus infection in guinea pig and swine

文献类型： 外文期刊

第一作者： Huang, HB

作者： Huang, HB;Yang, ZJ;Xu, QX;Sheng, ZT;Xie, Y;Yan, WY;You, YJ;Sun, LY;Zheng, ZX

作者机构：

期刊名称：VIRAL IMMUNOLOGY （ 影响因子：2.257； 五年影响因子：1.882 ）

ISSN： 0882-8245

年卷期： 1999 年 12 卷 1 期

页码：

收录情况： SCI

摘要： In this study, we provide evidence that a recombinant fusion protein containing P-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.

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