Odorant-Binding Protein 1 Plays a Crucial Role in the Olfactory Response of Bemisia tabaci to R-Curcumene

文献类型： 外文期刊

第一作者： Zhan, Haixia

作者： Zhan, Haixia;Tian, Jiahui;Li, Du;Qu, Cheng;Li, Fengqi;Luo, Chen;Dewer, Youssef;Zhang, Jinping;Yang, Zhen

作者机构：

关键词： Bemisia tabaci; Solanum habrochaites; odorant-binding proteins; competitive fluorescence binding assay; site-directed mutagenesis; RNA interference

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 43 期

页码：

收录情况： SCI

摘要： The cultivated tomato Solanum lycopersicum suffered a severe attack by the whitefly Bemisia tabaci (Gennadius), causing damage to leaves by feeding as well as transmitting the tomato yellow leaf curl virus (TYLCV), while the wild tomato S. habrochaites is considerably less appealing to this insect species. It is reported that B. tabaci shows innate avoidance to R-curcumene, which is produced naturally by S. habrochaites. However, the mechanisms involved in the avoidance behavior of B. tabaci in response to this chiral compound are still unclear yet. In this study, the functional and binding characterization of odorant-binding protein 1 of B. tabaci (BtOBP1) were examined in vivo and in vitro against R-curcumene. The obtained results showed that BtOBP1 exhibits specific binding activity to R-curcumene, which acts as repellents to B. tabaci. By using a fluorescence-based binding assay, the difference of binding-affinity for R-curcumene between wild type BtOBP1 and the mutant BtOBP1 to R-curcumene was performed, which resulted in a single amino acid mutation (ASN108 > SER); moreover, BtOBP1-N108 displays significantly decreased binding affinities to R-curcumene. Most interestingly, a knock-down experiment with the BtOBP1 showed that the whitefly responses to R-curcumene are impaired. This study illustrated that BtOBP I is a crucial protein involved in the perception and discrimination of R-curcumene. Our findings may provide an excellent chance of finding a suitable antagonist of eco-friendly features that can block the perception of chemosensory signals in insects, preventing behaviors like food-finding.

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