Determination of cordycepin content of Cordyceps militaris recombinant rice by high performance liquid chromatography

文献类型： 外文期刊

第一作者： Wang, Xuesong

作者： Wang, Xuesong;Liu, Fang;Li, Fang;Cai, Hongyan;Sun, Wei;Chen, Xuan;Shen, Wangyang;Li, Fang;Shen, Wangyang;Gao, Hong

作者机构：

关键词： Recombinant rice;Cordycepin;Chinese caterpillar fungus;Aweto;Cordyceps militaris

期刊名称：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH （ 影响因子：0.533； 五年影响因子：0.743 ）

ISSN： 1596-5996

年卷期： 2016 年 15 卷 10 期

页码：

收录情况： SCI

摘要： Purpose: To assess the suitability of high performance liquid chromatography (HPLC) for the determination of cordycepin content of Cordyceps militaris recombinant rice Methods: Cordyceps militaris recombinant rice was made by mixing brown rice with artificial Chinese caterpillar fungus culture medium powder using twin-screw extrusion technology. Cordycepin content was determined by reversed-phase HPLC with water: acetonitrile (95: 5, v/v) as mobile phase, detection wavelength of 260 nm, and flow rate of 1.0 mL/min. Results: Cordycepin contents showed good linearity in the range of 1 - 50.0 mu g/mL (r(2) = 0.9996), and while recovery ranged from 103.2 to 109.9 %. Relative standard deviation (RSD), precision and repeatability RSD was 2.38, 0.76 and 1.46 %, respectively. Conclusion: The HPLC method is simple, fast, accurate and reproducible. It is suitable for determination of cordycepin content of artificial Chinese caterpillar fungus culture medium and brown rice recombinant rice.

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