Identification of a gonad-expression differential gene insulin-like growth factor-1 receptor (Igf1r) in the swamp eel (Monopterus albus)

文献类型： 外文期刊

第一作者： Mei, Jie

作者： Mei, Jie;Fang, Jie;Yuan, Gailing;Chen, Nan;He, Yan;Mei, Jie;He, Yan;Mei, Jie;He, Yan;Yan, Wei

作者机构：

关键词： Igf1r;Swamp eel;Gonad differentiation;Hsp10

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN： 0920-1742

年卷期： 2014 年 40 卷 4 期

页码：

收录情况： SCI

摘要： In vertebrate species, the biopotential embryonic gonad differentiation is affected by many key genes and key steroidogenic enzymes. Insulin-like growth factor-1 receptor (Igf1r) has been considered as an important sex-differentiation gene in mammals and could mediate the biological action of Igf1, an important regulator of key steroidogenic enzymes. However, Igf1r gene is still unknown in the swamp eel, an economically important fish. In our study, we identified Igf1r gene in the swamp eel, which was a 2,148-bp open-reading frame encoding a protein of 716 amino acids. The alignment and the phylogenetic tree showed that Igf1r of the swamp eel had a conservative sequence with other vertebrates, especial fishes. Western blotting of Igf1r showed that Igf1r expressed much more in ovotestis and testis than in ovary, indicating an important role of Igf1r during gonad differentiation. We analyzed ubiquitination of Igf1r by co-immunoprecipitation and found the amount of ubiquitinated Igf1r was increased from ovary, ovotestis to testis, which was reversely to the trend of Hsp10 expression during gonadal transformation. It was possible that Hsp10 could suppress Igf1r ubiquitination during gonadal development of the swamp eel.

分类号：

  • 相关文献

[1]Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus. Li, Wei,Sun, Wen-xiu,Hong, Deng-wei,Meng, Liang.

[2]Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon. Shi, Jinxuan,Fu, Mingjun,Zhao, Chao,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Shi, Jinxuan,Fu, Mingjun,Zhao, Chao,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Yang, Qibin.

作者其他论文 更多>>

微信小程序