Development of elite rice restorer lines in the genetic background of R022 possessing tolerance to brown planthopper, stem borer, leaf folder and herbicide through marker-assisted breeding

文献类型： 外文期刊

第一作者： Wan, Bingliang

作者： Wan, Bingliang;Zha, Zhongping;Li, Jinbo;Xia, Mingyuan;Du, Xueshu;Yin, Desuo;Lin, Yongjun;Lin, Yongjun

作者机构：

关键词： bar;Bph14/Bph15;cry1C;Leaf folder resistance;Stem borer resistance;Oryza sativa L.

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2014 年 195 卷 1 期

页码：

收录情况： SCI

摘要： Rice leaf folder, stem borer and brown planthopper (BPH) are the most devastating rice insect pests. Developing and planting insect-resistant rice varieties is the most economical and effective measure for controlling these pests. BPH can be controlled with native BPH-resistance genes in rice, while at present rice leaf folder and stem borer can only be controlled through planting transgenic Bt rice. In this study, the breeding of a new restorer line KR022 possessing stacked BPH-resistance genes Bph14 and Bph15, Bt gene cry1C and glufosinate-resistance gene bar, is reported for the first time. A rice restorer line R022 with BPH-resistance genes Bph14 and Bph15 was used as a recurrent parent to cross with the transgenic rice T1C-19 of cry1C and bar genes during the breeding process. The restorer line KR022 was developed from the backcross populations of R022 and T1C-19 through molecular marker-assisted selection and glufosinate-resistance selection. The cry1C and bar genes were found to integrate on chromosome 11 of KR022, and the genome recovery of KR022 was up to 95.8 % of the R022 genome. The quantification of Cry1C protein expression showed that it was expressed at different levels in the leaf, stem, panicle, endosperm, and root of KR022 and its hybrid rice. The insect-resistance evaluation indicated that KR022 and its hybrid rice had good resistance to rice leaf folder and stem borer, both in laboratory settings and in the field. Furthermore, they exhibited increased resistance to BPH at both the seedling and mature stage. The field trial showed there was no significant difference in key agronomic traits between KR022 and its recurrent parent R022, and four hybrids from KR022 yield much higher than the control II-You 838. Moreover, KR022 and its hybrid rice were found to have resistance to the herbicide glufosinate. These results demonstrate that KR022 is effective as a rice restorer line for the breeding of "green super rice", possessing multiple tolerances to rice BPH, stem borer, leaf folder and glufosinate.

分类号：

  • 相关文献

[1]Economic Impact of Stem Borer-Resistant Genetically Modified Sugarcane in Guangxi and Yunnan Provinces of China. Yang, Ying-Ying,Xu, Li-Ping,Que, You-Xiong,Li, Yang-Rui. 2016

[2]No Direct Effects of Two Transgenic Bt Rice Lines, T1C-19 and T2A-1, on the Arthropod Communities. Han, N. S.,Hu, C.,Ye, G. Y.,Lu, Z. B.,Han, N. S.,Hu, C.,Ye, G. Y.,Tian, J. C.,Peng, Y. F.,Stanley, David.

[3]INGESTION OF Bt RICE POLLEN DOES NOTREDUCE THE SURVIVALORHYPOPHARYNGEAL GLAND DEVELOPMENT OF APIS MELLIFERA ADULTS. Chen, Xiuping,Romeis, Jorg,Peng, Yufa,Li, Yunhe,Wang, Yuanyuan,Chen, Xiuping,Romeis, Jorg,Peng, Yufa,Li, Yunhe,Wang, Yuanyuan,Shi, Jianrong,Dai, Pingli.

[4]Transgenic cry1C or cry2A rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Liu, Yu-E,Han, Nai-Shun,Hu, Cui,Ye, Gong-Yin,Lu, Zeng-Bin,Liu, Yu-E,Han, Nai-Shun,Hu, Cui,Ye, Gong-Yin,Tian, Jun-Ce,Peng, Yu-Fa,Guo, Yu-Yuan.

[5]Comparison of three transgenic Bt rice lines for insecticidal protein expression and resistance against a target pest, Chilo suppressalis (Lepidoptera: Crambidae). Ke, Kai-Qie,Li, Yun-He,Han, Lan-Zhi,Liu, Yan-Min,Peng, Yu-Fa,Ke, Kai-Qie,Hua, Hong-Xia. 2016

[6]Impacts of Bt Rice Expressing Cry1C or Cry2A Protein on the Performance of Nontarget Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), Under Laboratory and Field Conditions. Wang, W.,Hu, C.,Ye, G. Y.,Lu, Z. B.,Wang, W.,Hu, C.,Ye, G. Y.,Tian, J. C.,Xu, H. X.,Guo, Y. Y.,Peng, Y. F.. 2014

[7]Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries. Wang, Yun,Zhang, Xiao,Liu, Xianjin,Wang, Yun,Zhang, Xiao,Zhang, Cunzheng,Liu, Yuan,Liu, Xianjin.

[8]Crop production, nitrogen recovery and water use efficiency in rice-wheat rotation as affected by non-flooded mulching cultivation (NFMC). Ai, YW,Zhang, FS,Lu, SH,Zeng, XZ,Fan, MS. 2005

[9]Heritable alteration in DNA methylation induced by nitrogen-deficiency stress accompanies enhanced tolerance by progenies to the stress in rice (Oryza sativa L.). Von Wettstein, D.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Li, Y.,Song, X. X.,Ou, X. F.,Liu, B.,Kou, H. P.,Ma, J.,Xing, S. C.. 2011

[10]A simplified genomic DNA extraction protocol for pre-germination genotyping in rice. Duan, Y. B.,Zhao, F. L.,Chen, H. D.,Sheng, W.,Teng, J. T.,Zhang, A. M.,Xue, J. P.,Li, H.,Ni, D. H.,Wei, P. C.. 2015

[11]Inheritance of resistance to rice stripe virus in rice line 'BL 1'. Ise, K,Ishikawa, K,Li, CY,Ye, CR. 2002

[12]Genetic dissection of QTL against phosphate deficiency in the hybrid rice 'Xieyou9308'. Zhang, Yingxin,Anis, Galal Bakr,Wu, Weiming,Yu, Ning,Shen, Xihong,Zhan, Xiaodeng,Cheng, Shihua,Cao, Liyong,Wang, Ruci,Zhang, Yingxin,Wu, Weiming,Shen, Xihong,Zhan, Xiaodeng,Cheng, Shihua,Cao, Liyong,Anis, Galal Bakr. 2018

[13]Characterization and cloning of SMALL GRAIN 4, a novel DWARF11 allele that affects brassinosteroid biosynthesis in rice. Shi, Zhenyuan,Rao, Yuchun,Xu, Jie,Hu, Shikai,Fang, Yunxia,Yu, Haiping,Pan, Jiangjie,Liu, Ruifang,Ren, Deyong,Wang, Xiaohu,Zhu, Yangzhou,Zhu, Li,Dong, Guojun,Zhang, Guangheng,Zeng, Dali,Guo, Longbiao,Hu, Jiang,Qian, Qian,Rao, Yuchun,Zhu, Yangzhou,Xu, Jie. 2015

[14]Over-expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Shi, ZhenYing,Wang, Jiang,Wan, XinShan,Shen, GeZhi,Wang, XinQi,Zhang, JingLiu. 2007

[15]Cytoplasm and cytoplasm-nucleus interactions affect agronomic traits in japonica rice. Tao, DY,Hu, FY,Yang, JY,Yang, GF,Yang, YQ,Xu, P,Li, J,Ye, CR,Dai, LY. 2004

[16]Density alteration of nutrient elements in rice grains of a low phytate mutant. Ren, Xue-Liang,Liu, Qing-Long,Fu, Hao-Wei,Wu, Dian-xing,Shu, Qing-Yao. 2007

[17]Analysis of QTLs for panicle exsertion and its relationship with yield and yield-related traits in rice (Oryza sativa L.). Zhao, C. F.,Chen, T.,Zhao, Q. Y.,Zhou, L. H.,Zhao, L.,Zhang, Y. D.,Zhu, Z.,Yao, S.,Wang, C. L.. 2016

[18]Fine mapping and candidate gene analysis of a green-revertible albino gene gra(t) in rice. Chen, Tao,Zhang, Yadong,Zhao, Ling,Zhu, Zhen,Lin, Jing,Zhang, Suobing,Wang, Cailin. 2009

[19]An efficient and high-throughput protocol for Agrobacterium-mediated transformation based on phosphomannose isomerase positive selection in Japonica rice (Oryza sativa L.). Duan, Yongbo,Li, Hao,Li, Juan,Ni, Dahu,Song, Fengshun,Li, Li,Yang, Jianbo,Duan, Yongbo,Song, Fengshun,Zhai, Chenguang,Mei, Wenqian,Gui, Huaping,Zhang, Wanggen,Li, Hao,Li, Juan,Ni, Dahu. 2012

[20]Identification and Expression of Genes Involved in Race-specific Blast Resistance in Rice. Hu, Hai-Yan,Zheng, Kang-Le,Zhuang, Jie-Yun,Zheng, Kang-Le,Zhuang, Jie-Yun,Chai, Rong-Yao,Li, Yu-Chuan. 2010

作者其他论文 更多>>

微信小程序