Characterization of porcine SKIP gene in skeletal muscle development: Polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells

文献类型： 外文期刊

第一作者： Xiong, Qi

作者： Xiong, Qi;Liu, Yang;Suo, Xiaojun;Zhang, Nian;Li, Xiaofeng;Yang, Qianping;Chen, Mingxin;Chai, Jin;Deng, Changyan;Jiang, Siwen;Zheng, Rong;Chai, Jin;Deng, Changyan;Jiang, Siwen;Zheng, Rong;Huang, Tao

作者机构：

关键词： SKIP;Meat traits;Carcass traits;Pig;Cell proliferation;Cell cycle

期刊名称：MEAT SCIENCE （ 影响因子：5.209； 五年影响因子：5.305 ）

ISSN： 0309-1740

年卷期： 2012 年 92 卷 4 期

页码：

收录情况： SCI

摘要： Skeletal muscle and kidney-enriched inositol phosphatase (SKIP) was identified as a 5'-inositol phosphatase that hydrolyzes phosphatidylinositol (3,4,5)-triphosphate (PI(3,4,5)P3) to PI(3,4)P2 and negatively regulates insulin-induced phosphatidylinositol 3-kinase signaling in skeletal muscle. In this study, two new single nucleotide polymorphisms (SNPs) in porcine SKIP introns 1 and 6 were detected. The C1092T locus in intron 1 showed significant associations with some meat traits, whereas the A17G locus in intron 6 showed significant associations with some carcass traits. Expression analysis showed that porcine SKIP is upregulated at d 65 of gestation and Meishan fetuses have higher and prolonged expression of SKIP compared to Large White at d 100 of gestation. Ectopic expression of porcine SKIP decreased insulin-induced cell proliferation and promoted serum starvation-induced cell cycle arrest in G0/G1 phase in C2C12. Our results suggest that SKIP plays a negative regulatory role in skeletal muscle development partly by preventing cell proliferation. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved.

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