One-stop polymerase chain reaction (PCR): An improved PCR method with speedy operation and comparable efficiency

文献类型： 外文期刊

第一作者： Bi, Yan-zhen

作者： Bi, Yan-zhen;Zheng, Xin-min;Qiao, Xian-feng;Zhang, Jia-nong;Ou-yang, Hui-Wu;Ma, Jun

作者机构：

关键词： Polymerase chain reaction (PCR);post-cycling;compatible dye;Gelred

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2011 年 10 卷 83 期

页码：

收录情况： SCI

摘要： The widely used polymerase chain reaction (PCR) protocol requires several post-cycling steps to visualize amplicons, decelerating PCR sample processing and result calling. "One-stop PCR" was developed by including both the loading buffer and nontoxic staining dye within a single PCR tube, allowing direct loading and simultaneous staining of amplicons for visualization. The efficiency and sensitivity of one-stop PCR were comparable to commercially available PCR kits and it is compatible with downstream uses and is not mutagenic. One-stop PCR robustly minimizes the time and labor required for PCR reaction setup and product visualization.

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