Identification of an ethylene-responsive and cell wall-secreting β-1,3-glucanase, VvGLU1, in the early cell regrowth of grape winter buds triggered by exogenous dormancy releasers

文献类型： 外文期刊

第一作者： Shi, Zhaowan

作者： Shi, Zhaowan;Shi, Zhaowan;Wu, Jiamin;Mo, Hairuo;Pang, Xuequn;Shi, Zhaowan;Xue, Weiwen;Zhang, Zhaoqi

作者机构：

关键词： Vitis vinifera; Bud; Dormancy; Cell wall; beta-1,3-glucanase; Hydrogen cyanamide; Ethylene

期刊名称：BMC BIOLOGY （ 影响因子：4.5； 五年影响因子：5.4 ）

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年卷期： 2025 年 23 卷 1 期

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收录情况： SCI

摘要： BackgroundGrape (Vitis vinifera) crops encounter significant challenges in overcoming bud endodormancy in warm winter areas worldwide. Research on the mechanisms governing bud dormancy release has focused primarily on stress regulation; however, cell wall regulation of bud meristem regrowth mechanism during the dormancy release remains obscure.ResultsIn this study, transmission electron microscopy revealed significant changes in the grape bud cell wall following hydrogen cyanamide (HC) treatment, accompanied by an increase in beta-1,3-glucanase activity. We then investigated the potential contribution of beta-1,3-glucanases (GLUs) to the regulation of cell wall remodeling. Forty-eight VvGLUs distributed in clades alpha, beta, and gamma were identified and nominated based on the genome data of V. vinifera. Three gamma-clade VvGLUs (VvGLU1, VvGLU16, and VvGLU32) were upregulated by dormancy-releasing stimuli, including HC, sodium azide (AZ), ethylene and hypoxia. Among these, VvGLU1 presented increased gene transcription and protein expression in response to HC and ethylene treatment. The VvGLU1 promoter positively responded to ethylene, and its activity could be activated by VvERF57. Using both immunogold labeling and GFP fusion protein analysis, we observed that VvGLU1 localized in the endoplasmic reticulum, accumulated in the vacuole, and was secreted into the cell wall during HC-triggered dormancy release.ConclusionsBased on these findings, we propose that ethylene-regulated VvGLU1 plays a pivotal role in cell wall remodeling, thereby facilitating the regrowth of the bud meristem.

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