Multiple amino acid variations in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice

文献类型： 外文期刊

第一作者： Wu, Rui

作者： Wu, Rui;Tian, Yongxiang;Deng, Junhua;Yang, Keli;Liang, Wangwang;Guo, Rui;Duan, Zhengying;Liu, Zewen;Zhou, Danna;Xu, Diping;Wu, Rui;Tian, Yongxiang;Xu, Diping;Wu, Rui

作者机构：

关键词： Japanese encephalitis virus;Swine;Mouse;Adaptation;Pathogenicity

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2011 年 156 卷 4 期

页码：

收录情况： SCI

摘要： In a previous study, we performed serial brain-to-brain passages of swine Japanese encephalitis virus in mice and sequenced the complete genomes of the F5 and F20 passaged mouse-adapted variants. In the current study, we analyzed the differences between their genome sequences and found 12 amino acid substitutions in the nonstructural proteins. We also assessed the growth characteristics of these two variants in mammalian cells in vitro and in vivo. Our investigations revealed that the F20 variant had enhanced growth characteristics and modified virulence compared with the F5 variant. We therefore conclude that multiple amino acid substitutions in the nonstructural proteins of swine Japanese encephalitis virus alter its virulence in mice.

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