Identification of Self-Incompatibility Genotypes in Some Sand Pears (Pyrus pyrifolia Nakai) by PCR-RFLP Analysis

文献类型： 外文期刊

第一作者： Gu Qing-qing

作者： Gu Qing-qing;Zhang Qing-lin;Luo Zheng-rong;Gu Qing-qing;Hu Hong-jue;Chen Qi-liang

作者机构：

关键词： Pyrus pyrifolia Nakai;self-incompatibility genotype;S-allele;S-RNase;PCR-RFLP;crossing experiment

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 2 期

页码：

收录情况： SCI

摘要： The identification of self-incompatibility genotype (S-genotype) will be useful for selection of pollinizers and design of crossing in cultivar improvement of sand pear. This paper reported the identification of self-incompatibility genotypes of seven Chinese and two Japanese sand pear cultivars using PCR-RFLP analysis and S-RNase sequencing. The S-genotypes of these cultivars were determined as follows: Huali 1 S(1)S(3), Shounan S(1)S(3), Xizilu S(1)S(4), Qingxiang S(3)S(7), Sanhua S(2)S(7), Huangmi (Imamuranatsu) S(1)S(6), Huali 2 S(3)S(4), Baozhuli S(7)S(33), Cangxixueli S(5)S(15). S-RNase alleles (S(1)to S(9)) in sand pear could be identified effectively by PCR-RFLP analysis.

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