Efficient Re-Differentiation of Rice (Oryza sativa L.) Callus Induced by Exogenous Hormones Indole-3-Butytric Acid and Thidiazuron in Tissue Culture

文献类型: 外文期刊

第一作者: Lv, Junheng

作者: Lv, Junheng;Zhou, Junlei;Li, Ding;Cao, Mengliang;Li, Ding;Tang, Ning;Li, Ding;Cao, Mengliang

作者机构:

关键词: Tissue Culture;Exogenous Hormones;Re-Differentiation

期刊名称:JOURNAL OF BIOBASED MATERIALS AND BIOENERGY ( 影响因子:0.708; 五年影响因子:0.739 )

ISSN: 1556-6560

年卷期: 2017 年 11 卷 1 期

页码:

收录情况: SCI

摘要: Exogenous hormones play a significant role in the process of plant growth and tissue culture. The suitable hormones with reasonable ratio of concentration can effectively promote the proliferation and differentiation of rice callus. In this study, we explored the effects of 2,4-dichlorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA), indole-3-butytric acid (IBA) and 6-benzylaminopurine (6-BA), kinetin (KT) and thidiazuron (TDZ) on proliferation and re-differentiation of rice callus, and investigated their differences. Then, we chose IBA and TDZ for concentration ratio experiment to optimize hormone concentration for re-differentiation of rice callus. The results showed that 0.4 mg/L TDZ effectively promoted the re-differentiation of rice callus, and 2 mg/L IBA further improved the proliferation rate of rice callus. It has been well-known that appropriate auxin and cytokinin had a mutually reinforcing effect, so when the medium was added 0.5 mg/L TDZ and 2.5 mg/L IBA, the differentiation efficiency of rice callus could reach the optimized level.

分类号:

  • 相关文献

[1]Limited tissue culture-induced mutations and linked epigenetic modifications in F-1 hybrids of sorghum pure lines are accompanied by increased transcription of DNA methyltransferases and 5-methylcytosine glycosylases. von Wettstein, Diter,Zhang, Meishan,Xu, Chunming,Zhao, Na,Liu, Bao,Zhang, Meishan,Yan, Hongyan. 2009

[2]Molecular cytogenetic identification of Triticum aestivum Secale cereale substitution and addition lines. Li, HJ,Zhu, ZQ,Zhang, YM,Guo, BH,Wen, YX,Jia, X. 1998

[3]Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila). Chen, Jianzhong,Zhang, Haiyang,Wang, Chenyi,Zhou, Guoqing,Han, Zhiping,Chen, Jianzhong,Liu, Zhili,Dai, Jian. 2010

[4]Variation of B Chromosome Associated with Tissue Culture in Wheat-rye Cross. Li, Hongjie,Tian, Bohong. 2009

[5]Comparison of three ELISA methods for the detection of apple chlorotic leaf spot virus and apple stem grooving virus. Wu, YQ,Zhang, DM,Chen, SY,Wang, XF,Wang, WH. 1998

[6]Molecular cytogenetic analysis of intergeneric chromosomal translocations between wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture. Li, HJ,Guo, BH,Li, YW,Du, LQ,Jia, X,Chu, CC. 2000

[7]The Research on Actinidia eriantha Benth. in South China. Wu, Y. J.,Xie, M.,Zhang, Q. C.,Zhang, H. Q.,Fang, J. B.. 2011

[8]Investigation of metabolites accumulation in medical plant Gentiana rigescens during different growing stage using LC-MS/MS and FT-IR. Pan, Yu,Zhang, Ji,Zhao, Yan-Li,Wang, Yuan-Zhong,Pan, Yu,Huang, Heng-Yu. 2015

[9]Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice. Lin, Chunjing,Hu, Lanjuan,Yang, Jingjing,Zhou, Tianqi,Xu, Chunming,Liu, Bao,Lin, Chunjing,Lin, Xiuyun,Long, Likun,Xing, Shaochen,Dong, Yingshan,Qi, Bao. 2012

[10]Somatic Embryogenesis and Organogenesis of Biofuel Plant Cassava (Manihot esculenta Crantz) Chinese Cultivars. Li, Ruimei,Duan, Ruijun,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Guo, Jianchun,Fu, Shaoping,Li, Ruimei,Duan, Ruijun,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Guo, Jianchun,Fu, Shaoping,Ji, Yimeng,Xi, Dujuan,Liu, Jiao,Zhang, Peng. 2012

[11]Study on in vitro culture of mini-watermelon. Wang, X. Q.,You, H. B.. 2012

[12]Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips. Valkonen, J. P. T.,Wang, Q. C..

[13]Approaches to improve the transgenic efficiency and to rescue seedlings from hyperhydricity for rapeseed (Brassica napus). Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing,Ding, Yongqiang,Zhang, Dingyu,Yin, Guoying,Wang, Wenjing.

[14]HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana. Wang Hui,Wang Jiayuan,Wu Xiaopeng,Shen Haiyan,Luo Ying,Dai Haofu,Mei Wenli. 2015

[15]Caucasian clover (Trifolium ambiguum Bieb.) x white clover (T. repens L.) - interspecific hybrids developed through tissue culture. Huang, Fan,Wang, Mingjiu,Li, Zhiyong,Huang, Fan,Wang, Mingjiu. 2017

[16]Characterization and Comparative Expression Profiling of Browning Response in Medinilla formosana after Cutting. Wang, Yan,Wang, Yiting,Song, Xijiao,Chen, Jianping,Liz, Kunfeng. 2016

[17]Establishment of a high-efficiency plant regeneration and transformation system for the elite maize inbred lines from three heterotic groups. Liu, Xiaohong,Wu, Chunlian,Tan, Zhenbo,He, Daowen,Li, Linhui,Zhang, Hongmei. 2009

[18]Replication of pistillate plants of Ricinus communis L. and investigation of the sex stability and genetic variation of the somaclones. Tan, Meilian,Yan, Mingfang,Wang, Lei,Yan, Xingchu,Fu, Chunling,Wang, Lijun. 2013

[19]In Vitro Conservation and Cryopreservation in National Genebank of China. Zhang, Jinmei,Xin, Xia,Yin, Guangkun,Lu, Xinxiong,Chen, Xiaoling. 2014

[20]An protocol for genetic transformation of Catharanthus roseus by Agrobacterium rhizogenes A4. Zhou, Mei-Liang,Wu, Yan-Min,Tang, Yi-Xiong,Zhou, Mei-Liang,Shao, Ji-Rong,Zhu, Xue-Mei.

作者其他论文 更多>>