Using Hygromycin Phosphotransferase and Enhanced Green Fluorescent Protein Genes for Tracking Plastid Transformation in Rice (Oryza sativa L.) via Gold Particle Bombardment
文献类型: 外文期刊
第一作者: Tang, Ning
作者: Tang, Ning;Hu, Yuanyi;Xia, Yumei;Cao, Mengliang;Li, Ding;Li, Ding;Tang, Ning;Cao, Mengliang;Liu, Moxuan;Shen, Chunxiu;Cao, Mengliang
作者机构:
关键词: Plastid Transformation;Hygromycin Phosphotransferase;Enhanced Green Fluorescent Protein;Rice
期刊名称:NANOSCIENCE AND NANOTECHNOLOGY LETTERS ( 影响因子:1.128; 五年影响因子:0.985 )
ISSN: 1941-4900
年卷期: 2016 年 8 卷 5 期
页码:
收录情况: SCI
摘要: Plastid transformation has been successfully employed in several dicotyledonous plants for genetic modification, but it is difficult to be achieved in monocotyledonous cereal crops and remains still a demanding technique in the engineering agriculture field. One of major obstacles applying plastid transformation in crop plants is the limited availability of suitable selective markers. Hygromycin B is an aminoglycoside antibiotic which has the ability to inhibit prokaryotic protein synthesis, but to our best knowledge, it has never been reported in the research of plastid transformation yet. In this study, we aimed to use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice. A site-specific transformation vector of rice plastid, pCTE04, was constructed for dicistronic expression of the hygromycin phosphotransferase (hpt) and enhanced green fluorescent protein genes (EGFP). The results showed that transplastomic rice plants successfully grew up after a gradual selection of hygromycin resistance. Integration of the dicistronic expression cassette into the intergenic region trnI-trnA of plastid genome was verified by Southern blotting analysis. Transplastomic expression of the hpt and EGFP genes was identified by RT-PCR. The stable expression of dicistronic cassette in the rice was confirmed by confocal microscopy. Although the plastid transformants in rice presented a heteroplastomic status, this study may facilitate the development of new selective markers and further improvement of plastid transformation efficiency in cereal crops.
分类号:
- 相关文献
作者其他论文 更多>>
-
High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris
作者:Li, Bingkun;Zheng, Yiheng;Zhao, Shida;Zhang, Yaohan;Li, Ding;Li, Bingkun;Zheng, Yiheng;Zhao, Shida;Zhang, Yaohan;Li, Ding
关键词:E2-Spy; hybrid signal peptide cSP3; gene dosage optimization; molecular chaperone
-
One-line hybrid rice with high-efficiency synthetic apomixis and near-normal fertility
作者:Dan, Junhao;Zhan, Yijie;Tian, Junyou;Deng, Huafeng;Cao, Mengliang;Xia, Yumei;Tang, Ning;Cao, Mengliang;Wang, Yao;Cao, Mengliang;Cao, Mengliang
关键词:One-line hybrid rice; Apomixis; Multiple-embryos; Atavism
-
Research on Physiological Characteristics and Differential Gene Expression of Rice Hybrids and Their Parents under Salt Stress at Seedling Stage
作者:Zhang, Dan;Lei, Dongyang;Zhang, Dan;Hu, Yuanyi;Tang, Li;Mao, Bigang;Shao, Ye;Zhao, Bingran;Hu, Yuanyi;Li, Ruopeng;Mo, Lin;Pan, Yinlin;Hu, Yuanyi;Li, Ruopeng;Tang, Li;Mo, Lin;Mao, Bigang
关键词:rice; heterosis; salt tolerance; ROS; salt-related gene
-
Dietary copper requirement of broilers fed a corn-soybean meal diet during 22-42 d of age
作者:Zhu, Ling;Wu, Wei;Wu, Bingxin;Hu, Yun;Zhang, Weiyun;Li, Tingting;Cui, Xiaoyan;Gao, Feiyu;Li, Ding;Luo, Xugang;Wang, Shengchen;Zhang, Liyang
关键词:Copper; Copper content; Gene expression of copper -containing; enzyme; Requirement; Broiler
-
Identification and characterization of a fungal cutinase-like enzyme CpCut1 from Cladosporium sp. P7 for polyurethane degradation
作者:Liu, Jiawei;Xin, Kaiyuan;Zhang, Tianyang;Wen, Yuan;Zhou, Jie;Dong, Weiliang;Jiang, Min;Li, Ding;Wei, Ren;Zhou, Jie;Dong, Weiliang;Jiang, Min;Cui, Zhongli
关键词:polyurethane; fungal cutinase; biodegradation; Cladosporium sp.; polymer recycling
-
Iron Plaque: A Shield against Soil Contamination and Key to Sustainable Agriculture
作者:He, Zeping;Chen, Jinyuan;Yuan, Shilin;Chen, Sha;Zheng, Yi;Li, Ding;Chen, Sha;Li, Ding;Chen, Sha;Li, Ding;Hu, Yuanyi;Hu, Yuanyi
关键词:iron plaque; hydrophytes; heavy metals
-
A Universal Strategy for the Efficient Expression of Nanobodies in Pichia pastoris
作者:Zheng, Yiheng;Li, Bingkun;Zhao, Shida;Liu, Jiawei;Li, Ding;Zheng, Yiheng;Zhao, Shida;Li, Bingkun;Liu, Jiawei
关键词:model nanobodies; antibiotic-free parental plasmids; expression components; gene dosage; molecular chaperones; H55 host