Temperature and Development Impacts on Housekeeping Gene Expression in Cowpea Aphid, Aphis craccivora (Hemiptera: Aphidiae)

文献类型： 外文期刊

第一作者： Liu, Yong

作者： Liu, Yong;Yang, Chunxiao;Pan, Huipeng;Zhou, Xuguo

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 6 期

页码：

收录情况： SCI

摘要： Quantitative real-time PCR (qRT-PCR) is a powerful technique to quantify gene expression. To standardize gene expression studies and obtain more accurate qRT-PCR analysis, normalization relative to consistently expressed housekeeping genes (HKGs) is required. In this study, ten candidate HKGs including elongation factor 1 alpha (EF1A), ribosomal protein L11(RPL11), ribosomal protein L14(RPL14), ribosomal protein S8(RPS8), ribosomal protein S23(RPS23), NADH-ubiquinone oxidoreductase(NADH), vacuolar-type H+-ATPase (ATPase), heat shock protein 70(HSP70), 18S ribosomal RNA(18S), and 12S ribosomal RNA(12S) from the cowpea aphid, Aphis craccivora Koch were selected. Four algorithms, geNorm, Normfinder, BestKeeper, and the Delta C-t method were employed to evaluate the expression profiles of these HKGs as endogenous controls across different developmental stages and temperature regimes. Based on RefFinder, which integrates all four analytical algorithms to compare and rank the candidate HKGs, RPS8, RPL14, and RPL11 were the three most stable HKGs across different developmental stages and temperature conditions. This study is the first step to establish a standardized qRT-PCR analysis in A. craccivora following the MIQE guideline. Results from this study lay a foundation for the genomics and functional genomics research in this sap-sucking insect pest with substantial economic impact.

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