Optimizing Production of Pectinase from Orange Peel by Penicillium oxalicum PJ02 Using Response Surface Methodology
文献类型: 外文期刊
第一作者: Xia, Jin-Lan
作者: Xia, Jin-Lan;Nie, Zhen-Yuan;Gao, Qi-Rui;Ma, Ya-Long;Shan, Yang;Su, Dong-Lin;Shan, Yang;Su, Dong-Lin;Shan, Yang;Zhang, Chuang
作者机构:
关键词: Pectinase;Cellulolytic enzymes;Orange peel;Response surface methodology
期刊名称:WASTE AND BIOMASS VALORIZATION ( 影响因子:3.703; 五年影响因子:3.624 )
ISSN: 1877-2641
年卷期: 2015 年 6 卷 1 期
页码:
收录情况: SCI
摘要: A novel strain Penicillium oxalicum PJ02 was isolated for the production of exo-pectinase, endo-pectinase, carboxymethylcellulase (CMCase) and xylanase. Response surface methodology (RSM) was employed to optimize orange peel submerged fermentation for pectinase production. Plackett-Burman factorial design was used to select significant parameters. Temperature and NH4Cl concentration had significant influence on exo-pectinase production. The central composite design was used to generate a total of 22 fermentation experiments. The statistical results indicated that the optimum temperature for exo-pectinase and endo-pectinase production was 36.5 degrees C, and optimal NH4Cl concentration was 1.12 g/L. Under this condition, the yield of exo-pectinase was 36.88 U/mL, endo-pectinase was 0.62 U/mL, which are in close agreement with the values predicted by the model. The CMCase and xylanase yield in the same medium were 0.38 and 0.57 U/mL, respectively. RSM was also used to analyse the effect of independent variables on CMCase and xylanase production.
分类号:
- 相关文献
作者其他论文 更多>>
-
Bioinspired Janus starch film with dual functionality via citral nanoemulsion-mediated interfacial self-assembly for fresh-cut fruits and vegetables preservation
作者:Xie, Ying;Ding, Ke;Zhang, Shikai;Xu, Saiqing;Li, Huan;Lin, Shuhua;Shan, Yang;Ding, Shenghua;Xie, Ying;Ding, Ke;Xu, Saiqing;Shan, Yang;Ding, Shenghua;Sun, Yuying;Li, Yawen;Wang, Rongrong
关键词:Starch film; Janus structure; Rapid self-assembly; Fresh-cut fruits and vegetables preservation
-
Microbes: Drivers of Chenpi manufacturing, biotransformation, and physiological effects
作者:Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Fu, Yanjiao;Wang, Chao;Liao, Yanfang;Peng, Mingfang;Fu, Fuhua;Li, Gaoyang;Su, Donglin;Guo, Jiajing;Shan, Yang;Gao, Zhipeng
关键词:Chenpi; Manufacturing; Biotransformation; Physiological effects; Microbiome; Applications
-
Effects of rolling on eating quality, starch structure, and water distribution in cooked indica rice dough
作者:Xiong, Ying;Zhang, Yu;Yi, Cuiping;Zhu, Hong;Zhang, Yu;Shan, Yang;Fang, Zhongxiang
关键词:rolling process; cooked rice dough; sheeting; starch structure; water status
-
Delaying quality deterioration with multifunctional gelatin-based film by inhibiting microbial growth in fresh-cut navel oranges
作者:Xie, Ying;Xu, Saiqing;Ding, Ke;Xu, Haishan;Qiao, Xinbei;Tang, Qi;Shan, Yang;Ding, Shenghua;Xie, Ying;Xu, Saiqing;Ding, Ke;Xu, Haishan;Qiao, Xinbei;Tang, Qi;Wang, Zijun;Shan, Yang;Ding, Shenghua;Yang, Haiying;Wang, Rongrong;Wang, Zijun;Xu, Yanqun;Ding, Shenghua
关键词:Fresh-cut navel oranges; Multifunctional gelatin-based film; Flavor; Microbial community; Correlation analysis
-
Powerful combination: a genome editing system to improve efficiency of breeding inducer and haploid sorting in maize
作者:Xia, Hanchao;Yin, Yuejia;Zhang, Chuang;Chen, Ziqi;Liu, Xiangguo;Xia, Hanchao;Zhang, Di;Zhao, Rengui;Liu, Xiangguo;Qu, Yanzhi;Jiang, Shurong;Wang, Xinqi;Xu, Jieting
关键词:haploid; MoDsRed2; inducer line; genome-editing; Zea mays
-
Dynamic changes in the chemical structure and gelling properties of pectin at different stages of citrus maturation and storage
作者:Du, Yuyi;Zhao, Chengying;Wang, Jirong;Zheng, Jinkai;Bao, Yuming;Shan, Yang;Zheng, Jinkai
关键词:Citrus; Pectin; Maturity and storage; Chemical structure; Gelling properties
-
Engineering Saccharomyces cerevisiae for De Novo Biosynthesis of 3′-Hydroxygenistein
作者:Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Shan, Yang;Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Liu, Juan;Shan, Yang;Tan, Xinjia;Xiao, Zhiqiang;Zhang, Siqi;Wang, Yongtong;Zhao, Yifei;Lu, Qiyuan;Hu, Fanglin;Zuo, Shasha;Liu, Juan;Shan, Yang;Mao, Jiwei
关键词:3 '-hydroxygenistein; genistein; 2-hydroxyisoflavanonesynthase; Saccharomyces cerevisiae; metabolic engineering