Effect of Nitrogen Form on Gene Expression in Leaf Tissue of Greenhouse Grown Potatoes During Three Stages of Growth
文献类型: 外文期刊
第一作者: Tai, Helen
作者: Tai, Helen;De Koeyer, David;Li, Xiu-Qing;Luo, Sainan;Millard, Pete;Xiong, Xingyao
作者机构:
关键词: Nitrate;Ammonium;Nitrate transporter;Ammonium transporter;Potato;Solanum tuberosum
期刊名称:AMERICAN JOURNAL OF POTATO RESEARCH ( 影响因子:1.697; 五年影响因子:1.673 )
ISSN: 1099-209X
年卷期: 2012 年 89 卷 4 期
页码:
收录情况: SCI
摘要: This study examined the expression of genes in leaf tissue of greenhouse-grown potatoes to the form of N supplied in the context of development of a diagnostic tool for detection of N status. Potato cultivar 'Shepody' was grown over a two-week period at three stages of growth (tuber initiation, flowering and early tuber bulking) with three forms of N (NO3(-) only, NH4(+) only, or 1:1 NO3(-):NH4(+)) at two levels of N supply (0.75 or 7.5 mM). Leaf disks were collected and extracted for RNA, and 28 genes examined for expression using nCounter. Expression of ammonium transporter AT1 was increased when the N supply was reduced, regardless of N form, and was generally independent of growth stage. Expression of AT1 was less influenced by N form than currently used chemical or optical measures of plant N status. Expression of AT1 can be used as a quantitative indicator for plant N status of potato regardless of nitrogen form and crop growth stage.
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