Integrated DNA methylome and transcriptome analysis reveals the ethylene-induced flowering pathway genes in pineapple

文献类型： 外文期刊

第一作者： Wang, Jiabin

作者： Wang, Jiabin;Li, Zhiying;Lei, Ming;Fu, Yunliu;Zhao, Jiaju;Ao, Mengfei;Xu, Li;Wang, Jiabin;Li, Zhiying;Lei, Ming;Fu, Yunliu;Zhao, Jiaju;Ao, Mengfei;Xu, Li;Wang, Jiabin;Li, Zhiying;Lei, Ming;Fu, Yunliu;Zhao, Jiaju;Ao, Mengfei;Xu, Li

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Ethylene has long been used to promote flowering in pineapple production. Ethylene-induced flowering is dose dependent, with a critical threshold level of ethylene response factors needed to trigger flowering. The mechanism of ethylene-induced flowering is still unclear. Here, we integrated isoform sequencing iso-seq), Illumina short-reads sequencing and whole-genome bisulfite sequencing (WGBS) to explore the early changes of transcriptomic and DNA methylation in pineapple following high-concentration ethylene (HE) and low-concentration ethylene (LE) treatment. Iso-seq produced 122,338 transcripts, including 26,893 alternative splicing isoforms, 8,090 novel transcripts and 12,536 candidate long non-coding RNAs. The WGBS results suggested a decrease in CG methylation and increase in CHH methylation following HE treatment. The LE and HE treatments induced drastic changes in transcriptome and DNA methylome, with LE inducing the initial response to flower induction and HE inducing the subsequent response. The dose-dependent induction of FLOWERING LOCUS T-like genes (FTLs) may have contributed to dose-dependent flowering induction in pineapple by ethylene. Alterations in DNA methylation, lncRNAs and multiple genes may be involved in the regulation of FTLs. Our data provided a landscape of the transcriptome and DNA methylome and revealed a candidate network that regulates flowering time in pineapple, which may promote further studies.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序