Alterations of growth, antioxidant system and gene expression in Stylosanthes guianensis during Colletotrichum gloeosporioides infection

文献类型: 外文期刊

第一作者: Wang, Hui

作者: Wang, Hui;Jia, Yanxing;Luo, Lijuan;Chen, Zhijian;Liu, Guodao;Bai, Changjun;Qiu, Hong

作者机构:

关键词: Stylosanthes;Anthracnose;Colletotrichum gloeosporioides;Antioxidant system;Gene expression;Stress response

期刊名称:PLANT PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:4.27; 五年影响因子:4.816 )

ISSN: 0981-9428

年卷期: 2017 年 118 卷

页码:

收录情况: SCI

摘要: Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive fungal diseases of many plants, including stylo (Stylosanthes spp.), which is an important tropical forage legume. Although C. gloeosporioides-caused anthracnose is the major constraint limiting the growth and yield of stylo, little information is available regarding the responses of stylo during the infection process of this pathogen. This study investigated the changes in growth, the antioxidant system and gene expression in stylo in response to C. gloeosporioides treatment. Negative effects of C gloeosporioides were observed in inoculated stylo plants, as reflected by the formation of necrotic disease lesions and the decrease in shoot fresh weight. Reactive oxygen species (ROS) accumulation increased in stylo leaves during the C. gloeosporioides infection process. The activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase (GPX) and glutathione reductase (GR), as well as the concentrations of the antioxidant compounds ascorbate (AsA) and glutathione (GSH), increased in leaves under C gloeosporioides treatment. Furthermore, transcriptional analysis showed that the expression of stress response genes, including NADPH oxidase (Nox), thioredoxin (Thi), pathogenesis related genes (PR1 and PR5), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), chalcone synthase (CHS) and chitinase (Cht), was differentially enhanced in stylo leaves by C. gloeosporioides. Taken together, this study provides novel information regarding the alterations during the infection process of C gloeosporioides in stylo at the levels of antioxidant system and gene expression. (C) 2017 Elsevier Masson SAS. All rights reserved.

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