Efficient Sugarcane Transformation via bar Gene Selection

文献类型： 外文期刊

第一作者： Wang, W. Z.

作者： Wang, W. Z.;Yang, B. P.;Feng, C. L.;Wang, J. G.;Xiong, G. R.;Zhao, T. T.;Zhang, S. Z.

作者机构：

关键词： Sugarcane;Transgenic plant;Agrobacterium;Bar gene;Herbicide resistance

期刊名称：TROPICAL PLANT BIOLOGY （ 影响因子：1.512； 五年影响因子：2.011 ）

ISSN： 1935-9756

年卷期： 2017 年 10 卷 2-3 期

页码：

收录情况： SCI

摘要： Genetic engineering provides new opportunities for improving economically important traits in sugarcane cultivars. In this study, an efficient Agrobacterium-mediated transformation system that uses the bar gene (a herbicide resistance gene that is used in conjunction with the herbicide Basta) as a selection marker was developed. Using this transformation selection system, all of the resistant plants after selection were nearly 100% polymerase chain reaction (PCR) detection positive and showed herbicide resistance. Each gram of sugarcane calli used for transformation produced approximately 12 transgenic lines. It took approximately 4 months to generate transgenic plants that measured 10 cm in height for greenhouse transplantation.

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