Mechanism of internal browning of pineapple: The role of gibberellins catabolism gene (AcGA2ox) and GAs

文献类型： 外文期刊

第一作者： Zhang, Qin

作者： Zhang, Qin;Rao, Xiuwen;Zhang, Lubin;He, Congcong;Yang, Fang;Zhu, Shijiang;Zhang, Lubin

作者机构：

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： Internal browning (IB), a physiological disorder (PD) that causes severe losses in harvested pineapple, can be induced by exogenous gibberellins (GAs). Over the years, studies have focused on roles of Gibberellin 2-oxidase (GA2oxs), the major GAs catabolic enzyme in plants, in the regulation of changes in morphology or biomass. However, whether GA2oxs could regulate PD has not been reported. Here, a full-length AcGA2ox cDNA was isolated from pineapple, with the putative protein sharing 23.59% to 72.92% identity with GA2oxs from five other plants. Pineapples stored at 5 degrees C stayed intact, while those stored at 20 degrees C showed severe IB. Storage at 5 degrees C enhanced AcGA2ox expression and decreased levels of a GAs (GA4) 'compared with storage at 20 degrees C. However, at 20 degrees C, exogenous application of abscisic acid (ABA) significantly suppressed IB. ABA simultaneously upregulated AcGA2ox and reduced GA4. Ectopic expression of AcGA2ox in Arabidopsis resulted in reduced GA4, lower seed germination, and shorter hypocotyls and roots, all of which were restored by exogenous GA4/7. Moreover, in pineapple, GA4/7 upregulated polyphenol oxidase, while storage at 5 degrees C and ABA downregulated it. These results strongly suggest the involvement of AcGA2ox in regulation of GAs levels and a role of AcGA2ox in regulating IB.

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