Gain-of-function in Arabidopsis (GAINA) for identifying functional genes in Hevea brasiliensis

文献类型： 外文期刊

第一作者： Cheng, Han

作者： Cheng, Han;Gao, Jing;Cai, Haibin;Zhu, Jianshun;Huang, Huasun;Cheng, Han;Huang, Huasun

作者机构：

关键词： Gain-of-function;Rubber tree;Arabidopsis;Full-length cDNA library;Mutation;Forward genetics

期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）

ISSN： 2193-1801

年卷期： 2016 年 5 卷

页码：

收录情况： SCI

摘要： Background: Forward genetics approaches are not popularly applied in non-model plants due to their complex genomes, long life cycles, backward genetic studies etc. Researchers have to adopt reverse genetic methods to characterize gene functions in non-model plants individually, the efficiency of which is usually low. Results: In this study, we report a gain-of-function in Arabidopsis (GAINA) strategy which can be used for batch identification of functional genes in a plant species. This strategy aims to obtain the gain-of-function of rubber tree genes through overexpressing transformation ready full-length cDNA libraries in Arabidopsis. An initial transformation test produced about two thousand independent transgenic Arabidopsis lines, in which multiple obvious aberrant phenotypes were observed, suggesting the gain-of-function of rubber tree genes. The transferred genes were further isolated and identified. One gene identified to be metallothionein-like protein type 3 gene was further transferred into Arabidopsis and reproduced a similar aberrant phenotype. Conclusion: The GAINA system proves to be an efficient tool for batch identification of functional genes in Hevea brasiliensis, and also applicable in other non-model plants.

分类号：

  • 相关文献

[1]Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.). Liu, Qing-Long,Xu, Xiu-Hong,Ren, Xue-Liang,Fu, Hao-Wei,Wu, Dian-Xing,Shu, Qing-Yao.

[2]Identification, Functional Study, and Promoter Analysis of HbMFT1, a Homolog of MFT from Rubber Tree (Hevea brasiliensis). Bi, Zhenghong,Bi, Zhenghong,Huang, Huasun,Hua, Yuwei,Li, Xiang. 2016

[3]Cloning and characterization of a novel sulfate transporter gene from radish (Raphanus sativus L.). Wang, Zhi-Wei,Xiang, Chang-Ping,Mei, Shi-Yong.

[4]Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes. Zhao, Y. H.,Zhang, Z. B.,Zhao, C. Q.,Zhang, Y.,Wang, Y. F.,Zhu, Z. Q.,Guan, W. J.. 2015

[5]Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation. Tian, Jian,Wang, Ping,Gao, Shan,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu.

[6]De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree (Hevea brasiliensis Muell. Arg.). Li, Dejun,Deng, Zhi,Qin, Bi,Liu, Xianghong,Men, Zhonghua. 2012

[7]Rubber Trees Demonstrate a Clear Retranslocation Under Seasonal Drought and Cold Stresses. Li, Yuwu,Li, Yuwu,Lan, Guoyu,Xia, Yujie. 2016

[8]Design of natural rubber precision ditch fertilization machine. Wang, Yeqin,Deng, Yiguo,Zhang, Yuan,Wei, Lijiao. 2017

[9]Molecular mechanism of ethylene stimulation of latex yield in rubber tree (Hevea brasiliensis) revealed by de novo sequencing and transcriptome analysis. Liu, Jin-Ping,Zhuang, Yu-Fen,Guo, Xiu-Li,Li, Yi-Jian. 2016

[10]Identification of novel microRNAs in Hevea brasiliensis and computational prediction of their targets. Gebelin, Virginie,Argout, Xavier,Engchuan, Worrawat,Pitollat, Bertrand,Duan, Cuifang,Montoro, Pascal,Leclercq, Julie,Engchuan, Worrawat,Duan, Cuifang. 2012

[11]Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD). Liu, Jin-Ping,Tian, Xiao-Yan,Xia, Zhi-Qiang,Li, Yi-Jian. 2015

[12]Characterization of HbEREBP1, a wound-responsive transcription factor gene in laticifers of Hevea brasiliensis Muell. Arg.. Chen, Yue-Yi,Wang, Li-Feng,Dai, Long-Jun,Yang, Shu-Guang,Tian, Wei-Min,Chen, Yue-Yi.

[13]The small RNA profile in latex from Hevea brasiliensis trees is affected by tapping panel dryness. Gebelin, Virginie,Leclercq, Julie,Argout, Xavier,Sarah, Gautier,Montoro, Pascal,Kuswanhadi,Chaidamsari, Tetty,Hu, Songnian,Yang, Meng,Tang, Chaorong. 2013

[14]Ultrasound-assisted tapping of latex from Para rubber tree Hevea brasiliensis. She, Fenghua,Wang, Jin,An, Feng,Lin, Weifu,Zhu, Deming,She, Fenghua,Kong, Lingxue,An, Feng. 2013

[15]Characterization of Sugar Contents and Sucrose Metabolizing Enzymes in Developing Leaves of Hevea brasiliensis. Zhu, Jinheng,Qi, Jiyan,Fang, Yongjun,Xiao, Xiaohu,Lan, Jixian,Tang, Chaorong,Zhu, Jinheng,Lan, Jixian,Tang, Chaorong,Li, Jiuhui. 2018

[16]Next-generation sequencing, assembly, and comparative analyses of the latex transcriptomes from two elite Hevea brasiliensis varieties. Li, Dejun,Liu, Hui,Zhao, Manman,Deng, Zhi,Li, Yu,Zeng, Rizhong,Tian, Weimin,Hao, Lili,Zhao, Manman. 2015

[17]Proteome analysis of interaction between rootstocks and scions in Hevea brasiliensis. Yuan, Kun,Yang, Li-Fu,Wang, Zhen-Hui,Lin, Wei-Fu,Cao, Jian-Hua,Yuan, Kun,Yang, Li-Fu,Wang, Zhen-Hui,Lin, Wei-Fu,Cao, Jian-Hua,Ding, Xuan. 2011

[18]Regulation of MIR Genes in Response to Abiotic Stress in Hevea brasiliensis. Gebelin, Virginie,Leclercq, Julie,Montoro, Pascal,Hu, Songnian,Tang, Chaorong. 2013

[19]Molecular cloning and characterization of S-adenosylmethionine decarboxylase gene in rubber tree (Hevea brasiliensis). Zhao, Manman,Liu, Hui,Deng, Zhi,Chen, Jiangshu,Yang, Hong,Li, Dejun,Zhao, Manman,Li, Huiping,Xia, Zhihui.

[20]Development, characterization, genetic diversity and cross-species/genera transferability of ILP markers in rubber tree (Hevea brasiliensis). Li, Dejun,Deng, Zhi,Xia, Zhihui,Liu, Xianghong,Feng, Fuying.

作者其他论文 更多>>

微信小程序