Evaluation of four protein extraction methods for proteomic analysis of mango peel

文献类型： 外文期刊

第一作者： Liao, D. J.

作者： Liao, D. J.;Lu, X. P.;Chen, H. S.;Mo, Z. Y.;Lu, Y.

作者机构：

关键词： Mango peel;Protein extraction;2-DE;Proteomic analysis;Evaluation

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 3 期

页码：

收录情况： SCI

摘要： The peel of mango (Mangifera indica L.) is a special plant tissue that contains many compounds that interfere with protein extraction. A successful separation with Two-dimensional electrophoresis (2-DE) is the key step for proteomic analysis. To evaluate the efficiencies of mango peel protein extraction for 2-DE, four extraction methods were tested: 1) 2-D clean-up kit, 2) trichloroacetic acid/acetone precipitation, 3) phenol extraction, 4) phenol with methanol/ammonium acetate precipitation. The results showed that the phenol with methanol/ammonium acetate precipitation produced the best quality protein extraction and separation. Proteins were separated in 30-70 and > 70 kDa ranges better than with the other methods. Acidic proteins had better resolution with fewer horizontal and vertical streaks. Sixteen proteins were identified by maxtrix-assisted laser desorption/ionisation time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS/MS). The result demonstrated that each of these four methods can be used to prepare mango peel proteins. The phenol with methanol/ammonium acetate precipitation was the best choice for proteomic analysis of mango peel.

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