Genetic diversity in the germplasm of black pepper determined by EST-SSR markers

文献类型： 外文期刊

第一作者： Wu, B. D.

作者： Wu, B. D.;Fan, R.;Hu, L. S.;Wu, H. S.;Hao, C. Y.;Wu, B. D.;Fan, R.;Hu, L. S.;Wu, H. S.;Hao, C. Y.

作者机构：

关键词： Black pepper;Germplasms;EST-SSR;Polymorphism;Genetic diversity

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 1 期

页码：

收录情况： SCI

摘要： This study aimed to assess genetic diversity in the germplasm of black pepper from around the world using SSR markers from EST. In total, 13 markers were selected and successfully amplified the target loci across the black pepper germplasm. All the EST-SSR markers showed high levels of polymorphisms with an average polymorphism information content of 0.93. The genetic similarity coefficients among all accessions ranged from 0.724 to 1.000, with an average of 0.867. These results indicated that black pepper germplasms possess a complex genetic background and high genetic diversity. Based on a cluster analysis, 148 black pepper germplasms were grouped in two major clades: the Neotropics and the Asian tropics. Peperomia pellucida was grouped separately and distantly from all other accessions. These results generally agreed with the genetic and geographic distances. However, the Asian tropics clade did not cluster according to their geographic origins. In addition, compared with the American accessions, the Asian wild accessions and cultivated accessions grouped together, indicating a close genetic relationship. This verified the origin of black pepper. The newly developed EST-SSRs are highly valuable resources for the conservation of black pepper germplasm diversity and for black pepper breeding.

分类号：

  • 相关文献

[1]AFLP Analysis on the Genetic Diversity of Two Hundred Accessions of Mango in China. Gao, A. P.,Chen, Y. Y.,Zhu, M.,Huang, J. F.,Luo, H. Y.,Gao, A. P.,He, Y. H.,Jonathan, H. C..

[2]EST sequencing and SSR marker development from cultivated peanut (Arachis hypogaea L.). Li, Meng Jun,Xiao, Han,Wang, Xing Jun,Xia, Han,Zhao, Chuan Zhi,Bi, Yu Ping,Tang, Rong Hua. 2010

[3]Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis. Sha, Z-X.,Luo, X-H.,Liao, X-L.,Wang, Q-L.,Chen, S-L.,Wang, S-L.,Wang, S-L.. 2011

[4]Development of EST-SSR in foxtail millet (Setaria italica). Jia, Xiao-Ping,Shi, Yun-Su,Song, Yan-Cun,Wang, Guo-Ying,Wang, Tian-Yu,Li, Yu. 2007

[5]The development of EST-SSR markers in Lilium regale and their cross-amplification in related species. Yuan, Suxia,Ge, Liang,Liu, Chun,Ming, Jun.

[6]Development of EST-SSR markers related to salt tolerance and their application in genetic diversity and evolution analysis in Gossypium. B.H. Wang,P. Zhu,Y.L. Yuan,C.B. Wang,C.M. Yu,H.H. Zhang,X.Y. Zhu,W. Wang,C.B. Yao,Z.M. Zhuang,P. Li. 2014

[7]High-throughput development of simple sequence repeat markers for genetic diversity research in Crambe abyssinica. Qi, Weicong,Lin, Feng,Zhao, Han,Liu, Yuhe,Huang, Bangquan,Cheng, Jihua,Zhang, Wei. 2016

[8]Genetic diversity and relationships among 47 loquat varieties revealed by EST-SSR markers. Li, Xiao-ying,Xu, Hong-xia,Chen, Jun-wei. 2013

[9]Genetic Diversity Analysis of Faba Bean (Vicia faba L.) Based on EST-SSR Markers. Gong Ya-ming,Xu Sheng-chun,Hu Qi-zan,Zhang Gu-wen,Ding Ju,Mao Wei-hua,Li Ze-yun. 2011

[10]Diversity distribution and population structure of tea germplasms in China revealed by EST-SSR markers. Yao, Ming-Zhe,Ma, Chun-Lei,Qiao, Ting-Ting,Jin, Ji-Qiang,Chen, Liang. 2012

[11]Core EST-SSR Marker Selection Based on Genetic Linkage Map Construction and Their Application in Genetic Diversity Analysis of Litchi (Litchi chinensis Sonn.) Germplasm Resources. Xiang, X.,Chen, D. M.,Ma, S. P.,Ma, W. C.,Fan, J.,Yang, X. Y.,Liu, M.,Sun, Q. M.. 2014

[12]Genetic diversity and relationships among different tomato varieties revealed by EST-SSR markers. Korir, N. K.,Tao, R.,Li, X.,Kayesh, E.,Li, A.,Zhen, W.,Diao, W.,Wang, S.,Li, X.. 2014

[13]Genetic diversity and evolutionary relationship analyses within and among Raphanus species using EST-SSR markers. Wang, Qingbiao,Zhang, Li,Zheng, Pengjing,Wang, Qingbiao,Zhang, Li,Zheng, Pengjing.

[14]Comparison of genetic diversity and growth traits among Fangzheng silver crucian carp (Carassius auratus gibelio) gynogenetic clones. Lu, C. Y.,Cheng, L.,Li, C.,Zheng, X. H.,Cao, D. C.,Sun, X. W.. 2016

[15]Development of EST-SSR markers to study genetic diversity in hyacinth bean (Lablab purpureus L.). Zhang, Guwen,Xu, Shengchun,Gong, Yaming,Hu, Qizan,Mao, Weihua. 2013

[16]DEVELOPMENT AND CHARACTERIZATION OF 21 EST-DERIVED MICROSATELLITE MARKERS IN VICIA FABA (FAVA BEAN). Ma, Yu,Yang, Tao,Guan, Jianping,Wang, Shumin,Wang, Haifei,Sun, Xuelian,Zong, Xuxiao.

[17]Examining genetic relationships of Chinese Pleurotus ostreatus cultivars by combined RAPD and SRAP markers. Yin, Yonggang,Liu, Yu,Wang, Shouxian,Zhao, Shuang,Xu, Feng,Yin, Yonggang. 2013

[18]Development of SSR molecular markers for Allium mongolicum. Hu, Jing,Hu, Xiaoke,Zhang, Jinhu,Fan, Baoli,Yu, Qiushi,Zhang, Qian.

[19]Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Zhang, Haiying,Wang, Hui,Guo, Shaogui,Ren, Yi,Gong, Guoyi,Xu, Yong,Weng, Yiqun.

[20]Genetic diversity among germplasms of Pitaya based on SSR markers. Fu, Jiaxin,Zhang, Rong,Qin, Yonghua,Lu, Fang,Jia, Lili,Hu, Qinglei,Liu, Chengming,Pan, Limei,Huang, Lifang,Liang, Guidong.

作者其他论文 更多>>

微信小程序