Ultra-performance convergence chromatography (UPC2) method for the analysis of biogenic amines in fermented foods

文献类型： 外文期刊

第一作者： Gong, Xiao

作者： Gong, Xiao;Qi, Ningli;Lin, Lijing;Li, Jihua;Wang, Xiaoxi

作者机构：

关键词： Ultra-performance convergence chromatography (UPC2);Diode array detector;Biogenic amines;Determination;Fermented foods

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2014 年 162 卷

页码：

收录情况： SCI

摘要： A rapid ultra-performance convergence chromatography (UPC2) method for the determination of eight biogenic amines (spermine, spermidine, putrescine, cadaverine, tryptamine, phenylethylamine, histamine and tyramine) in fermented foods was developed. The amines were pre-column derivatized with dansyl chloride and separated on UPC2 system with a HSS C-18 SB column (3.0 x 100 mm, 1.8 mu m) using gradient elution with a binary system of CO2 and n-hexane:isopropanol:ammonium hydroxide (70:30:0.15, v/v/v), back pressure of 2000 psi, flow rate of 2.0 ml/min and DAD detection at 254 nm. The result showed excellent linearity (r = 0.9995-1.0000). Limits of detection (LOD) and limits of quantification (LOQ) were 21-67 ng/L and 72-224 ng/L, respectively. Relative standard deviations (RSD) for repeatability and reproducibility were 0.21-0.87% and 1.98-4.02%, respectively. Furthermore, this method was successfully applied to analysis of biogenic amines in Yulu and Sufu samples. (C) 2014 Elsevier Ltd. All rights reserved.

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