Effect of rapid freezing-thawing techniques on the sperm parameters and ultrastructure of Chinese Taihang black goat spermatozoa

文献类型: 外文期刊

第一作者: Shi, Liguang

作者: Shi, Liguang;Zhou, Hanlin;Hou, Guanyu;Xun, Wenjuan;Shi, Liguang;Ren, Youshe;Xun, Wenjuan;Yue, Wenbin;Yang, Rujie

作者机构:

关键词: Spermatozoa;Freeze-thawing process;Transmission electron microscopy;Computer-assisted semen analysis;Ultrastructure

期刊名称:MICRON ( 影响因子:2.251; 五年影响因子:1.969 )

ISSN: 0968-4328

年卷期: 2014 年 57 卷

页码:

收录情况: SCI

摘要: Supercooling sperm in liquid nitrogen vapour is a feasible and economic technique for the practical production. The study aimed to reveal the negative effects of this rapid freezing and thawing processes on Taihang black goat spermatozoa and to find out the changing of spermatozoa motility and ultrastructure by using CASA and TEM. Qualified semen samples, which collected from twenty Chinese Taihang black goats using artificial vagina were pooled and investigated the kinematics parameters and ultrastructural morphology. The results showed that freezing-thawing caused a significant reduction in the spermatozoon total motility (P< 0.001), in rapid and medium cell numbers (P< 0.001) and motility parameters (VAP, VSL, VCL, ALH and BCF) (P< 0.01). Immotile spermatozoa number was increased significantly after freezing-thawing (P <0.001). In the ultrastructural analysis, the shape with a sperm nucleus characterized by ruptures, bend and deformity was observed. The plasma membranes were broken, and nucleoplasm erupted. The mitochondria in the middle piece were disturbed by partial absence or additional accumulations. Swelling, coiling, vacuolization and structural disorganization of mitochondria were also observed. In conclusion, Freezing-thawing procedure has a detrimental effect on motility, membrane integrity and mitochondria of goat spermatozoa. Transmission electron microscopy provides an intuitive observation to investigate deformity spermatozoa. (C) 2013 Elsevier Ltd. All rights reserved.

分类号:

  • 相关文献

[1]Ultrastructure of Chemosensilla on Antennae and Tarsi of Ectropis obliqua (Lepidoptera: Geometridae). Bian, Lei,Li, Zhao-Qun,Cai, Xiao-Ming,Luo, Zong-Xiu,Chen, Zong-Mao,Ma, Long.

[2]Cryopreservation of flounder (Paralichthys olivaceus) sperm with a practical methodology. Zhang, YZ,Zhang, SC,Liu, XZ,Xu, YY,Wang, CL,Sawant, MS,Li, J,Chen, SL.

[3]Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization. Chen, SL,Ji, XS,Yu, GC,Tian, YS,Sha, ZX.

[4]Is transcription in sperm stationary or dynamic?. Ren, Xiaoxia,Chen, Xiaoli,Wang, Dong,Wang, Zhenling.

[5]Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi. Ding, Shuyan,Ge, Jiachun,Hao, Chen,Zhang, Mingsheng,Yan, Weihui,Xu, Zhiqiang,Pan, Jianlin,Chen, Songlin,Tian, Yongsheng,Huang, Yahong.

[6]Utilization and Metabolism of a-Starch by Black Carp (Mylopharyngodon piceus) Juveniles. Miao Ling-Hong,Ge Xian-Ping,Xie Jun,Zhu Jian,Liu Bo,Zhou Qun-Lan,Miao Ling-Hong,Ren Ming-Chun,Ge Xian-Ping,Xie Jun,Zhu Jian,Liu Bo,Zhou Qun-Lan,Pan Liang-Kun. 2016

[7]Green synthesis of gold nanoparticles using Citrus maxima peel extract and their catalytic/antibacterial activities. Yuan, Chun-Gang,Huo, Can,Gui, Bing,Cao, Wei-Ping. 2017

[8]Effects of external phosphorus on the cell ultrastructure and the chlorophyll content of maize under cadmium and zinc stress. Jiang, H. M.,Yang, J. C.,Zhang, J. F..

[9]Male gamete development and early tapetal degeneration in cytoplasmic mate-sterile pepper investigated by meiotic, anatomical and ultrastructural analyses. Luo, X. -D.,Dai, L. -F.,Wang, S. -B.,Wolukau, J. N.,Jahn, M.,Chen, J. -F.. 2006

[10]Splenic necrosis signs and pathogen detection in cultured half-smooth tongue sole, Cynoglossus semilaevis Gunther. Xiao, P.,Sun, M.,Zhang, L.,Xiao, P.,Liu, Y.,Zhang, L.,Xiao, P.,Jiang, M.,Jie, L.,Li, G.,Mo, Z..

[11]Purification of the recombinant hepatitis B virus core antigen (rHBcAg) produced in the yeast Saccharomyces cerevisiae and comparative observation of its particles by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Chen, H,Lu, JH,Liang, WQ,Huang, YH,Zhang, WJ,Zhang, DB. 2004

[12]Salicylic Acid Enhances Resistance to Fusarium oxysporum f. sp. phaseoli in Common Beans (Phaseolus vulgaris L.). Xue, Ren Feng,Wu, Jing,Wang, Lan Fen,Wang, Xiao Ming,Zhu, Zhen Dong,Wang, Shu Min,Xue, Ren Feng,Ge, Wei De,Blair, Matthew W.. 2014

[13]Isolation and characterization of a herpesvirus from feral pigeons in China. Zhao, Panpan,Ma, Jian,Guo, Ying,Tian, Li,Guo, Guangyang,Zhang, Kexin,Xing, Mingwei,Ma, Jian. 2015

[14]Reduced Glutathione Mediates Phone-Ultrastrcture, Kinome and Transportome in Chromium-Induced Brassica napus L.. Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Mao, Bizeng,Ali, Basharat,Tong, Chaobo,Liu, Shengyi. 2017

[15]An electrochemiluminescence aptasensor switch for aldicarb recognition via ruthenium complex-modified dendrimers on multiwalled carbon nanotubes. Li, Shuhuai,Liu, Chunhua,Han, Bingjun,Luo, Jinhui,Yin, Guihao,Li, Shuhuai,Liu, Chunhua,Han, Bingjun,Luo, Jinhui,Yin, Guihao. 2017

[16]Antibody-free colorimetric determination of total aflatoxins by mercury(II)-mediated aggregation of lysine-functionalized gold nanoparticles. Du, Bibai,Xiao, Can,Zhou, Ying,Wu, Li,Zhao, Hong,Yang, Jingkui,He, Yujian,Wang, Peilong,Su, Xiaoou,Wu, Li,He, Yujian.

[17]Perception of potential sex pheromones and host-associated volatiles in the cotton plant bug, Adelphocoris fasciaticollis (Hemiptera: Miridae): morphology and electrophysiology. Liu, Ze-Wen,Sun, Liang,Xiao, Hai-Jun,Gu, Shao-Hua,Guo, Yu-Yuan,Zhang, Yong-Jun,Xiao, Hai-Jun. 2014

[18]Uptake and cellular distribution, in four plant species, of fluorescently labeled mesoporous silica nanoparticles. Sun, Dequan,Hussain, Hashmath I.,Cahill, David M.,Sun, Dequan,Yi, Zhifeng,Kong, Lingxue,Siegele, Rainer,Cresswell, Tom.

[19]Morphology of unfertilized mature and fertilized developing marine pelagic eggs in four types of multiple spawning flounders. Bian, Xiaodong,Zhang, Xiumei,Gao, Tianxiang,Wan, Ruijing,Chen, Siqing,Sakurai, Yasunari. 2010

[20]Photochemical and antioxidative responses of the glume and flag leaf to seasonal senescence in wheat. Kong, Lingan,Sun, Mingze,Xie, Yan,Wang, Fahong,Zhao, Zhendong,Sun, Mingze. 2015

作者其他论文 更多>>