Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis

文献类型： 外文期刊

第一作者： Zhai, W.

作者： Zhai, W.;Zhao, Y.;Zhang, L. X.;Zhai, W.;Li, X. J.

作者机构：

关键词： Disease resistance gene;Pto;Serine/threonine kinase;Hevea brasiliensis

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 2 期

页码：

收录情况： SCI

摘要： The tomato Pto gene encodes a serine/threonine kinase (STK) whose molecular characterization has provided valuable insights into the disease resistance mechanism of tomato. Therefore, Pto is considered as a promising candidate for engineering broad-spectrum pathogen resistance in this crop. In this study, a pair of degenerate primers based on conserved subdomains of plant STKs similar to the tomato Pto protein was used to amplify similar sequences in a hevea cultivar (Hevea brasiliensis Muell. Arg). A fragment of similar to 550 bp was amplified, cloned, and sequenced. The sequence analysis of several clones revealed 12 distinct sequences highly similar to STKs. Based on their significant similarity with the tomato Pto protein (BLASTX E value <3e-53), seven sequences were classified as Pto resistance gene candidates (Pto-RGCs). Multiple sequence alignment of the hevea Pto-RGC products revealed that these sequences contain several conserved subdomains present in most STKs, as well as several conserved residues that are crucial for Pto function. Moreover, phylogenetic analysis showed that the hevea Pto-RGCs clustered with Pto, suggesting a common evolutionary origin with this resistance gene. The Pto-RGCs isolated in this study represent a valuable sequence resource that could assist in the development of disease resistance in hevea.

分类号：

  • 相关文献

[1]Identification, Phylogeny, and Expression Analysis of Pto-like Genes in Pepper. Wan, Hongjian,Yuan, Wei,Ruan, Meiying,Ye, Qingjing,Wang, Rongqing,Li, Zhimiao,Zhou, Guozhi,Yao, Zhuping,Yang, Yuejian.

[2]The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis. Zhang, Chunyan,Sun, Wen,Dong, Mengmeng,Liu, Wanquan,Li, Lu,Xu, Zhuofei,Zhou, Rui,Tan, Meifang,Gao, Ting,Li, Lu,Xu, Zhuofei,Zhou, Rui. 2017

[3]ISOLATION AND CHARACTERIZATION OF A POLLEN-SPECIFIC GENE ZMSTK2_USP FROM ZEA MAYS. Javeed, A.,Fan, M. X.,Zhang, C. Y.,Ma, W. J.,Chen, M. M.,Liu, K. C.,Cai, F. C.,Lin, F.,Shi, L.,Hussain, K.. 2018

[4]Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique. Luo, M,Kong, XY,Jiang, T,Jia, C,Zhou, RH,Jia, JZ.

[5]Genome-wide comparative analysis of NBS-encoding genes between Brassica species and Arabidopsis thaliana. Yu, Jingyin,Tehrim, Sadia,Zhang, Fengqi,Tong, Chaobo,Huang, Junyan,Cheng, Xiaohui,Dong, Caihua,Zhou, Yanqiu,Hua, Wei,Liu, Shengyi,Zhou, Yanqiu,Qin, Rui. 2014

[6]Phytohormone and genome variations in Vitis amurensis resistant to downy mildew. Qu, Junjie,Lu, Jiang,Deng, Shuhan,Liu, Shaoli,Zhang, Yali,Lu, Jiang. 2017

[7]Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis. Wang, Ying,Zhan, Di-Feng,Li, Hui-Liang,Guo, Dong,Zhu, Jia-Hong,Peng, Shi-Qing,Zhan, Di-Feng. 2017

[8]Three MADS-box genes of Hevea brasiliensis expressed during somatic embryogenesis and in the laticifer cells. Li, Hui-Liang,Wang, Ying,Guo, Dong,Peng, Shi-Qing,Tian, Wei-Min. 2011

[9]Acyl-CoA-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of Hevea brasiliensis (the Para rubber tree). Nie, Zhiyi,Wang, Yihang,Wu, Chuntai,Li, Yu,Kang, Guijuan,Qin, Huaide,Zeng, Rizhong,Nie, Zhiyi,Wang, Yihang,Wu, Chuntai,Li, Yu,Kang, Guijuan,Qin, Huaide,Zeng, Rizhong,Wang, Yihang. 2018

[10]The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families in Hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression. Xiao, Xiao-Hu,Sui, Jin-Lei,Qi, Ji-Yan,Fang, Yong-Jun,Tang, Chao-Rong,Yang, Meng,Hu, Song-Nian,Sui, Jin-Lei. 2017

[11]Molecular cloning, expression profiles and characterization of a novel translationally controlled tumor protein in rubber tree (Hevea brasiliensis). Li, Dejun,Deng, Zhi,Liu, Xianghong,Qin, Bi,Liu, Xianghong. 2013

[12]Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis. Cheng, Han,Chen, Xiang,Zhu, Jianshun,Huang, Huasun. 2016

[13]A convenient and efficient protocol for isolating high-quality RNA from latex of Hevea brasiliensis (para rubber tree). Tang, Chaorong,Qi, Jiyan,Li, Heping,Zhang, Cunliang,Wang, Yuekun. 2007

[14]Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones. Tong, Zheng,Wang, Dan,Sun, Yong,Yang, Qian,Meng, Xueru,Wang, Limin,Wang, Xuchu,Feng, Weiqiang,Wang, Xuchu,Li, Ling,Wurtele, Eve Syrkin,Wang, Xuchu,Li, Ling,Wurtele, Eve Syrkin,Li, Ling. 2017

[15]Characterization of HbWRKY1, a WRKY transcription factor from Hevea brasiliensis that negatively regulates HbSRPP. Wang, Ying,Guo, Dong,Li, Hui-Liang,Peng, Shi-Qing,Wang, Ying,Peng, Shi-Qing. 2013

[16]Laticifer differentiation in Hevea brasiliensis: Induction by exogenous jasmonic acid and linolenic acid. Hao, BZ,Wu, JL. 2000

[17]Molecular mechanism of ethylene stimulation of latex yield in rubber tree (Hevea brasiliensis) revealed by de novo sequencing and transcriptome analysis. Liu, Jin-Ping,Zhuang, Yu-Fen,Guo, Xiu-Li,Li, Yi-Jian. 2016

[18]Genome-wide identification and expression analysis of the metacaspase gene family in Hevea brasiliensis. Liu, Hui,Deng, Zhi,Li, Dejun,Chen, Jiangshu,Wang, Sen,Hao, Lili.

[19]Differential gene expression profiles in latex from Hevea brasiliensis between self-rooting juvenile and donor clones. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing.

[20]Effects of powdery mildew infection on chloroplast and mitochondrial functions in rubber tree. Wang, Li-Feng,Wang, Meng,Zhang, Yu.

作者其他论文 更多>>

微信小程序