Detection of Papaya leaf distortion mosaic virus by reverse-transcription loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Shen, Wentao

作者： Shen, Wentao;Tuo, Decai;Yan, Pu;Li, Xiaoying;Zhou, Peng;Shen, Wentao;Tuo, Decai;Yan, Pu;Li, Xiaoying;Zhou, Peng;Tuo, Decai

作者机构：

关键词： Papaya leaf distortion mosaic virus;PLDMV detection;Reverse-transcription loop-mediated isothermal amplification;RT-PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2014 年 195 卷

页码：

收录情况： SCI

摘要： Papaya leaf distortion mosaic virus (PLDMV) can infect transgenic papaya resistant to a related pathogen, Papaya ringspot virus (PRSV), posing a substantial threat to papaya production in China. Current detection methods, however, are unable to be used for rapid detection in the field. Here, a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of PLDMV, using a set of four RT-LAMP primers designed based on the conserved sequence of PLDMV CP. The RT-LAMP method detected specifically PLDMV and was highly sensitive, with a detection limit of 1.32 x 10(-6) mu g of total RNA per reaction. Indeed, the reaction was 10 times more sensitive than one-step RT-PCR, while also requiring significantly less time and equipment. The effectiveness of RT-LAMP and one-step RT-PCR in detecting the virus were compared using 90 field samples of non-transgenic papaya and 90 field samples of commercialized PRSV-resistant transgenic papaya from Hainan Island. None of the non-transgenic papaya tested positive for PLDMV using either method. In contrast, 19 of the commercialized PRSV-resistant transgenic papaya samples tested positive by RT-LAMP assay, and 6 of those tested negative by RT-PCR. Therefore, the PLDMV-specific RT-LAMP is a simple, rapid, sensitive, and cost-effective tool in the field diagnosis and control of PLDMV. (C) 2013 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Comparison of conventional RT-PCR, reverse-transcription loop-mediated isothermal amplification, and SYBR green I-based real-time RT-PCR in the rapid detection of bovine viral diarrhea virus nucleotide in contaminated commercial bovine sera batches. Zhang, Shu-Qin,Tan, Bin,Li, Peng,Wang, Feng-Xue,Guo, Li,Yang, Yong,Sun, Na,Zhu, Hong-Wei,Wen, Yong-Jun,Cheng, Shi-Peng.

[2]Complete genome sequence of an isolate of papaya leaf distortion mosaic virus from commercialized PRSV-resistant transgenic papaya in China. Tuo, D.,Li, Ch,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Tuo, D.,Shen, W.,Yan, P.,Gao, L.,Li, X.,Zhou, P.,Li, H.. 2013

[3]Simultaneous detection of papaya ringspot virus, papaya leaf distortion mosaic virus, and papaya mosaic virus by multiplex real-time reverse transcription PCR. Huo, P.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P..

[4]Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV. Chen, Changmu,Cui, Shangjin,Zhang, Chaofan,Li, Jun,Wang, Jinbao. 2010

[5]Genetic variability in Grapevine virus A from Vitis vinifera L. x Vitis labrusca L. in Sichuan, China. Xi, Dehui,Yuan, Shu,Lin, Honghui,Wang, Jianhui,Chen, Keling,Li, Hongwen,Liu, Xiao,Wang, Jianhui,Liu, Jianjun,Chen, Keling,Li, Hongwen,Liu, Xiao,Liu, Jianjun,Ercisli, Sezai. 2012

[6]Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification. Li, Changlong,Chen, Jianfei,Shi, Hongyan,Zhang, Xin,Shi, Da,Han, Xiao,Feng, Li,Chi, Yanbin. 2014

[7]LMOf2365_0442 Encoding for a Fructose Specific PTS Permease IIA May Be Required for Virulence in L-monocytogenes Strain F2365. Liu, Yanhong,Yoo, Brian B.,Hwang, Cheng-An,Huang, Lihan,Suo, Yujuan,Sheen, Shiowshuh,Khosravi, Parvaneh. 2017

[8]Development of an RT-PCR detection method for mud crab reovirus. Guo, Zhi-Xun,Weng, Shao-Ping,Li, Guang,He, Jian-Guo,Guo, Zhi-Xun,Chan, Siu-Ming. 2008

[9]Protease-activated receptor-2 (PAR-2) regulates enterotoxigenic Escherichia coli-induced diarrhea during weaning in piglets. Pei, Xiao-ying,Guo, Ding-zong,Zhou, Dong-hai,Gao, Hong-Mei,Xia, Tian,Guo, Rui. 2010

[10]Rapid detection of newly isolated Tembusu-related Flavivirus by reverse-transcription loop-mediated isothermal amplification assay. Wang, Youling,Liao, Ming,Wang, Youling,Yuan, Xiaoyuan,Li, Yufeng,Yu, Kexiang,Yang, Jinxing,Xu, Huaiying,Zhang, Yuxia,Qin, Zhuoming,Yu, Kangzhen. 2011

[11]Estimating Plasmodiophora brassicae gene expression in lines of B. rapa by RT-PCR. Wu, Li-yan,Li, Shi-kai,Gong, Ya-ju,Zhong, Li,He, Jiang-Ming,Siemens, Johannes,Ludwig-Mueller, Jutta. 2012

[12]Cloning and expression analysis of a partial LEAFY homologue from pineapple (Ananas comosus (L.) Merr.). Lv Lingling,Sun Guangming,Zhang Jianxia,Zeng Songjun,Duan Jun,Lv Lingling,Xie Jianghui,Liu Shenghui,Liu Yuge,Wei Changbin,Lv Lingling,Sun Guangming. 2011

[13]The incidence and molecular characteristics of Apple stem grooving virus from pear in China. Hu, G. -J.,Wang, G. -P.,Wang, L. -P.,Hong, Ni.,Hu, G. -J..

[14]Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Jian Gui-Liang,Zhang Ying-Tao,Ai Tie-Min.

[15]Identification and characterization of a novel adenine phosphoribosyltransferase gene (ZmAPT2) from maize (Zea mays L.). Wu, Suowei,Yu, Zhanwang,Li, Weihua,Yang, Qingkai,Ye, Chunjiang,Sun, Yan,Jin, Demin,Wang, Bin,Wu, Suowei,Li, Weihua,Yang, Qingkai,Wang, Fengge,Zhao, Jiuran.

[16]Self-priming on the plant viral RNAs during reverse transcription-PCR. Zhang, C.,Wu, H. N.,Zhang, Y. Q.,Li, W. M.,Shen, J. G..

[17]Transcription factor MdCBF1 gene increases freezing stress tolerance in transgenic Arabidopsis thaliana. Xue, Y.,Wang, Y. Y.,Peng, R. H.,Zhen, J. L.,Zhu, B.,Gao, J. J.,Zhao, W.,Han, H. J.,Yao, Q. H..

[18]Expression of the GPRC5D gene in Liaoning Cashmere goats. Jin, Mei,Li, XiuNa,Piao, Jun,Qu, YangLe,Wang, YiMeng,Piao, JingAi,Chu, MingXing.

[19]Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis. Ye, Huan,Du, Hao,Chen, Xi-Hua,Liu, Tao,Li, Chuang-Ju,Ye, Huan,Liu, Tao,Du, Hao,Li, Chuang-Ju,Cao, Hong.

[20]Comparison of apoptosis between adult worms of Schistosoma japonicum from susceptible (BALB/c mice) and less-susceptible (Wistar rats) hosts. Wang, Tao,Guo, Xiaoyong,Hong, Yang,Han, Hongxiao,Cao, Xiaodan,Han, Yanhui,Zhang, Min,Wu, Miaoli,Fu, Zhiqiang,Lu, Ke,Li, Hao,Zhao, Zhixin,Lin, Jiaojiao,Han, Yanhui,Zhang, Min,Lin, Jiaojiao.

作者其他论文 更多>>

微信小程序