Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant

文献类型： 外文期刊

第一作者： Zhang, Chao

作者： Zhang, Chao;Fu, Shaoping;Guo, Jianchun;Zhang, Chao;Zhang, Chao;Tang, Gaijuan;Hu, Xinwen

作者机构：

关键词： Explants;Jatropha curcas;Mature leaf;Plant growth regulators;Regeneration;Thidiazuron

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）

ISSN： 1054-5476

年卷期： 2013 年 49 卷 5 期

页码：

收录情况： SCI

摘要： In order to establish a highly efficient and sustainable regeneration system, we systematically researched the key factors affecting direct shoot regeneration from Jatropha curcas leaves that were collected from Hainan (HN1-1), Lijiang (LJ3-1), and Yuxi (YX2-12) provinces in China. The L-9(3(4)) orthogonal test of thidiazuron (TDZ), kinetin (Kn), and gibberellic acid (GA(3)) were studied, and the explant type, growth age, and cultivar of leaves were subsequently investigated. Simultaneously, the combinations of plant growth regulators (PGRs) promoting shoot bud proliferation, elongation, and root establishment were examined. The results showed that the best medium for shoot bud induction was Murashige and Skoog (MS) medium supplemented with 1.0 mg/L TDZ, 0.5 mg/L Kn, and 0.5 mg/L GA(3). TDZ was the key PGR, while Kn and GA(3) played an important role in shoot bud elongation and the number of shoots per leaf disk, respectively. The induced shoot buds proliferated and readily elongated in MS medium with 0.3 mg/L 6-benzylaminopurine and 0.01 mg/L indole-3-butyric acid (IBA) and established roots in half-strength MS medium supplemented with 2.0 mg/L IBA. Using the previously described methods, the third to fifth leaves were found to be the best explant source for shoot bud induction, with a high induction rate, large shoot numbers per disk, excellent proliferation, and consistent rooting. With the use of this regeneration system, the shoot bud induction rate increased from the reported rate of 53.5% to more than 90% using different explants and cultivars, and the shoot number per leaf disk (shoot length a parts per thousand yenaEuro parts per thousand 0.5 cm) increased from 1.6 to 3.5. Thus, this optimized regeneration system will effectively promote the propagation and genetic transformation of J. curcas.

分类号：

  • 相关文献

[1]Plantlet Regeneration of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) in Vitro Tissue Cultures. Wang, Cheng-Long,Dong, Xue-ni,Ding, Meng-qi,Shao, Ji-Rong,Wang, Cheng-Long,Dong, Xue-ni,Ding, Meng-qi,Tang, Yi-Xiong,Wu, Yan-Min,Zhou, Mei-Liang,Zhu, Xue-Mei.

[2]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[3]An efficient shoot regeneration system and Agrobacterium-mediated transformation with codA gene in a doubled haploid line of broccoli. Sheng, Xiaoguang,Gu, Honghui,Yu, Huifang,Wang, Jiansheng,Zhao, Zhenqing,Qi, Zirong. 2016

[4]The Influence of Thidiazuron on Proliferation of Acer x freemanii In Vitro. Zhao, X. Q.,Yuan, Z. H.,Yin, Y. L.,Feng, L. J.,Yin, Y. L.,Xu, R.. 2012

[5]In vitro propagation of the endangered tree fern Cibotium barometz through formation of green globular bodies. Yu, Rongpei,Li, Han,Cao, Hua,Mo, Xijun,Gui, Min,Zhou, Xuhong,Jiang, Yalian,Li, Shenchong,Wang, Jihua,Yu, Rongpei,Li, Han,Cao, Hua,Mo, Xijun,Gui, Min,Zhou, Xuhong,Jiang, Yalian,Li, Shenchong,Wang, Jihua,Zhang, Guangfei.

[6]Changes in endogenous hormones and H2O2 burst during shoot organogenesis in TDZ-treated Saussurea involucrate explants. Guo, Bin,He, Wei,Wu, Yandi,Fu, Yanping,Wei, Yahui,Zhao, Yan,Zhao, Yan,Guo, Juan.

[7]Quantitative determination of phorbol ester derivatives in Chinese Jatropha curcas seeds by high-performance liquid chromatography/mass spectrometry. Li, Li,Li, Wenqing,Chen, Fang,Li, Jing,Lu, Daihua,Chen, Fang,Li, Jing. 2013

[8]Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield. Xia, Zhiqiang,Zhang, Shengkui,Wen, Mingfu,Lu, Cheng,Sun, Yufang,Zou, Meiling,Wang, Wenquan,Xia, Zhiqiang,Zhang, Shengkui,Zou, Meiling. 2018

[9]Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis. Chen, Y.,Zhou, B.,Zhou, L. R.,Cai, J.,Yang, F. X.. 2015

[10]Genetic variation and phylogenetic relationship analysis of Jatropha curcas L. inferred from nrDNA ITS sequences. Guo, Guo-Ye,Chen, Fang,Shi, Xiao-Dong,Tian, Yin-Shuai,Guo, Guo-Ye,Yu, Mao-Qun,Han, Xue-Qin,Yuan, Li-Chun,Zhang, Ying.

[11]IMPROVEMENT ON THE THERMOSTABILITY AND ACTIVITY OF APX1 FROM ENERGY PLANT JATROPHA CURCAS L. BY HYPER-ACIDIC FUSION PARTNERS. Zhang, Mengru,Yang, Yumei,Li, Xujuan,Wang, Haibo,Yang, Shuanglong,Wang, Shasha,Gong, Ming,Zou, Zhurong,Li, Xujuan,Wang, Haibo. 2014

[12]Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing. 2014

[13]Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas. Wei, Qian,Li, Jun,Zhang, Lin,Wu, Pingzhi,Chen, Yaping,Li, Meiru,Jiang, Huawu,Wu, Guojiang,Wei, Qian,Zhang, Lin,Li, Jun. 2012

[14]Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners. Zhang, Mengru,Gong, Ming,Yang, Yumei,Zou, Zhurong,Li, Xujuan,Wang, Haibo.

[15]Effects of Calcium and Calmodulin Antagonists on Chilling Stress-Induced Proline Accumulation in Jatropha curcas L.. Yang, Shuang-Long,Deng, Feng-Fei,Gong, Ming,Lan, Shan-Shan.

[16]Molecular and functional characterization of the JcMYB1, encoding a putative R2R3-MYB transcription factor in Jatropha curcas. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing.

[17]Impact of genotype, plant growth regulators and activated charcoal on embryogenesis induction in microspore culture of pepper (Capsicum annuum L.). Cheng, Yan,Ma, Rong-li,Jiao, Yan-sheng,Qiao, Ning. 2013

[18]Simultaneous Determination of Five Plant Growth Regulators in Fruits by Modified Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) Extraction and Liquid Chromatography-Tandem Mass Spectrometry. Shi, Xiaomei,Jin, Fen,Huang, Yuting,Du, Xinwei,Wang, Miao,Shao, Hua,Jin, Maojun,Wang, Jing,Li, Chunmei.

[19]Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry (Fragaria x ananassa cv. 'Honeoye'). Wang, Hua,Li, Maofu,Yang, Yuan,Dong, Jing,Jin, Wanmei.

[20]POTENTIAL OF PLANT GROWTH REGULATOR AND CHLORMEQUAT CHLORIDE ON ALFALFA SEED COMPONENTS. Chen, Ji-Shan,Zhu, Rui-Fen,Lin, Hong,Han, Weibo,Ma, Li-Gong. 2016

作者其他论文 更多>>

微信小程序