Molecular and biochemical characterization of a cyanogenic beta-glucosidase in the inner bark tissues of rubber tree (Hevea brasiliensis Muell. Arg.)

文献类型： 外文期刊

第一作者： Tian, Wei-Min

作者： Tian, Wei-Min;Yang, Shu-Guang;Shi, Min-Jing;Dai, Long-Jun;Chen, Yue-Yi;Zhang, Hua;Wang, Xu-Chu

作者机构：

关键词： Hevea brasiliensis Muell. Arg.;Cyanogenic glucosidase;Latex regeneration;Protein-storing cell;Tapping

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2013 年 170 卷 8 期

页码：

收录情况： SCI

摘要： Tapping causes the loss of large amounts of latex from laticifers and subsequently enhances latex regeneration, a high carbon- and nitrogen-cost activity in rubber tree. It is suggested that a 67 kDa protein associated with protein-storing cells in the inner bark tissues of rubber tree plays an important role in meeting the nitrogen demand for latex regeneration. Here, the 67 kDa protein was further characterized by a combination of cell biological, molecular biological and biochemical techniques. Immunogold labeling showed that the 67 kDa protein was specifically localized in the central vacuole of protein-storing cells. A full-length cDNA, referred to as HbVSP1, was cloned. The HbVSP1 contained a 1584 bp open reading frame encoding a protein of 527 amino acids. The putative protein HbVSP1 shared high identity with the P66 protein from rubber tree and proteins of the linamarase, and bg1 A from cassava (Manihot esculenta). HbVSP1 contained the active site sequences of beta-glucosidase, TFNEP and I/VTENG. In vitro analysis showed that the 67 kDa protein exhibited the activity of both beta-glucosidase and linamarase and was thus characterized as a cyanogenic P-glucosidase. Proteins immuno-related to the 67 kDa protein were present in leaves and lutoids of laticifers. Tapping down-regulated the expression of HbVSP1, but up-regulated the expression of genes encoding the key enzymes for rubber biosynthesis, while the effect of resting from tapping was the reverse. Taken together, the results suggest that the 67 kDa protein is a vacuole-localized cyanogenic beta-glucosidase encoded by HbVSP1 and may have a role in nitrogen storage in inner bark tissues of trunk during the leafless periods when rubber tree is rested from tapping. (C) 2013 Elsevier GmbH. All rights reserved.

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