Molecular cloning, expression profiles and characterization of a novel translationally controlled tumor protein in rubber tree (Hevea brasiliensis)

文献类型： 外文期刊

第一作者： Li, Dejun

作者： Li, Dejun;Deng, Zhi;Liu, Xianghong;Qin, Bi;Liu, Xianghong

作者机构：

关键词： Expression analyses;Hevea brasiliensis;DNA protection activity;HbTCTP1;TPD

期刊名称：JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：3.549； 五年影响因子：4.164 ）

ISSN： 0176-1617

年卷期： 2013 年 170 卷 5 期

页码：

收录情况： SCI

摘要： The translationally controlled tumor protein (TCTP) is a multi-functioning protein that carries out vital roles in various life processes. In this study, a new TCTP gene, designated as HbTCTP1, was isolated in Hevea brasiliensis. The full-length complementary DNA (cDNA) of HbTCTP1 contained a maximum open reading frame (ORF) of 507 base pair (bp) encoding 168 amino acids. The sequence comparison showed that the deduced HbTCTP1 indicated high identities to plant TCTP proteins, and clustered in the dicot cluster of plant TCTPs. Although HbTCTP1 and human TCTP proteins did not parallel in overall sequence similarity, they indicated highly similar 3D structures with a nearly identical spatial organization of alpha-helices, beta-sheets, and coil regions. Real time reverse-transcription PCR (RT-PCR) analyses showed that HbTCTP1 was expressed throughout different tissues and developmental stages of leaves. Besides being related to tapping panel dryness (TPD), the HbTCTP1 transcripts were regulated by various treatments, including drought, low temperature, high salt, ethrel (ET), wounding, H2O2, and methyl jasmonate (Me-JA) treatments. The recombinant HbTCTP1 fusion protein was shown to protect supercoiled plasmid DNA from damages induced by metal-catalyzed generation of reactive oxygen species. The Ca-45(2+)-overlay assay showed that HbTCTP1 was a calcium-binding protein. Our results are greatly helpful in understanding the molecular characterization and expression profiles of HbTCTP1, and lay the foundation for further analyzing the function of HbTCTP1 in rubber tree. (C) 2012 Elsevier GmbH. All rights reserved.

分类号：

  • 相关文献

[1]Expression Profiles, Characterization and Function of HbTCTP in Rubber Tree (Hevea brasiliensis). Deng, Zhi,Chen, Jiangshu,Liu, Changren,Liu, Hui,Yang, Hong,Li, Dejun,Chen, Jiangshu,Liu, Changren,Xia, Zhihui,Leclercq, Julie,Montoro, Pascal,Zhou, Zhuangzhi,Zhou, Zhuangzhi. 2016

[2]Molecular cloning and expression analysis of the mevalonate diphosphate decarboxylase gene from the latex of Hevea brasiliensis. Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong,Wu, Chuntai,Li, Yu,Nie, Zhiyi,Dai, Longjun,Kang, Guijuan,Zeng, Rizhong.

[3]Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis. Chen, SC,Peng, SQ,Huang, GX,Wu, KX,Fu, XH,Chen, ZQ.

[4]Transcriptome sequencing and analysis of rubber tree (Hevea brasiliensis Muell.) to discover putative genes associated with tapping panel dryness (TPD). Liu, Jin-Ping,Tian, Xiao-Yan,Xia, Zhi-Qiang,Li, Yi-Jian. 2015

[5]Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis. Wang, Ying,Zhan, Di-Feng,Li, Hui-Liang,Guo, Dong,Zhu, Jia-Hong,Peng, Shi-Qing,Zhan, Di-Feng. 2017

[6]Three MADS-box genes of Hevea brasiliensis expressed during somatic embryogenesis and in the laticifer cells. Li, Hui-Liang,Wang, Ying,Guo, Dong,Peng, Shi-Qing,Tian, Wei-Min. 2011

[7]Acyl-CoA-binding protein family members in laticifers are possibly involved in lipid and latex metabolism of Hevea brasiliensis (the Para rubber tree). Nie, Zhiyi,Wang, Yihang,Wu, Chuntai,Li, Yu,Kang, Guijuan,Qin, Huaide,Zeng, Rizhong,Nie, Zhiyi,Wang, Yihang,Wu, Chuntai,Li, Yu,Kang, Guijuan,Qin, Huaide,Zeng, Rizhong,Wang, Yihang. 2018

[8]The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families in Hevea brasiliensis-comparison with five other plant species in structure, evolution, and expression. Xiao, Xiao-Hu,Sui, Jin-Lei,Qi, Ji-Yan,Fang, Yong-Jun,Tang, Chao-Rong,Yang, Meng,Hu, Song-Nian,Sui, Jin-Lei. 2017

[9]Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis. Cheng, Han,Chen, Xiang,Zhu, Jianshun,Huang, Huasun. 2016

[10]A convenient and efficient protocol for isolating high-quality RNA from latex of Hevea brasiliensis (para rubber tree). Tang, Chaorong,Qi, Jiyan,Li, Heping,Zhang, Cunliang,Wang, Yuekun. 2007

[11]Comparative Proteomics of Rubber Latex Revealed Multiple Protein Species of REF/SRPP Family Respond Diversely to Ethylene Stimulation among Different Rubber Tree Clones. Tong, Zheng,Wang, Dan,Sun, Yong,Yang, Qian,Meng, Xueru,Wang, Limin,Wang, Xuchu,Feng, Weiqiang,Wang, Xuchu,Li, Ling,Wurtele, Eve Syrkin,Wang, Xuchu,Li, Ling,Wurtele, Eve Syrkin,Li, Ling. 2017

[12]Characterization of HbWRKY1, a WRKY transcription factor from Hevea brasiliensis that negatively regulates HbSRPP. Wang, Ying,Guo, Dong,Li, Hui-Liang,Peng, Shi-Qing,Wang, Ying,Peng, Shi-Qing. 2013

[13]Laticifer differentiation in Hevea brasiliensis: Induction by exogenous jasmonic acid and linolenic acid. Hao, BZ,Wu, JL. 2000

[14]Molecular mechanism of ethylene stimulation of latex yield in rubber tree (Hevea brasiliensis) revealed by de novo sequencing and transcriptome analysis. Liu, Jin-Ping,Zhuang, Yu-Fen,Guo, Xiu-Li,Li, Yi-Jian. 2016

[15]Genome-wide identification and expression analysis of the metacaspase gene family in Hevea brasiliensis. Liu, Hui,Deng, Zhi,Li, Dejun,Chen, Jiangshu,Wang, Sen,Hao, Lili.

[16]Differential gene expression profiles in latex from Hevea brasiliensis between self-rooting juvenile and donor clones. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing.

[17]Effects of powdery mildew infection on chloroplast and mitochondrial functions in rubber tree. Wang, Li-Feng,Wang, Meng,Zhang, Yu.

[18]Age-dependent and jasmonic acid-induced laticifer-cell differentiation in anther callus cultures of rubber tree. Tan, Deguan,Sun, Xuepiao,Zhang, Jiaming. 2014

[19]The SWEET gene family in Hevea brasiliensis - its evolution and expression compared with four other plant species. Sui, Jin-Lei,Sui, Jin-Lei,Xiao, Xiao-Hu,Qi, Ji-Yan,Fang, Yong-Jun,Tang, Chao-Rong,Sui, Jin-Lei,Xiao, Xiao-Hu,Qi, Ji-Yan,Fang, Yong-Jun,Tang, Chao-Rong. 2017

[20]Molecular characterization and expression analysis of cDNAs encoding four Rab and two Arf GTPases in the latex of Hevea brasiliensis. Qin, Yunxia,Shi, Feng,Tang, Chaorong,Qin, Yunxia,Shi, Feng,Tang, Chaorong,Shi, Feng.

作者其他论文 更多>>

微信小程序