Cloning and Sequence Analysis of Two cDNA Encoding invertase Inhibitors from Cassava (Manihot esculenta Crantz)

文献类型： 外文期刊

第一作者： Geng, Mengting

作者： Geng, Mengting;Yao, Yuan;Wu, Xiaohui;Fu, Shaoping;Guo, Jianchun

作者机构：

关键词： Cassava;Invertase inhibitor;Gene cloning

期刊名称：ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6

ISSN： 1022-6680

年卷期： 2013 年 726-731 卷

页码：

收录情况： SCI

摘要： In this study, two encoding invertase inhibitors (NH) named as MeINH1 and MeINH2 were isolated from cassava (Manihot esculenta Crantz). The MeINH1 contains a 583 bp fragment in length and codes a region of 172 amino acids; and the MeINH2 contains a 538 bp fragment in length and codes a region of 169 amino acids. The amino acid sequence analysis showed that both MeINH1 and MeINH2 have typical structure of invertase inhibitor proteins with a N-terminal signal peptide, four conserved cysteine residues and two disulfide bridges, respectively; whereas, they showed low homology to other organisms. This research provides a foundation for further study of the cassava INH.

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