RNAi silencing of the Meloidogyne incognita Rpn7 gene reduces nematode parasitic success

文献类型： 外文期刊

第一作者： Niu, Junhai

作者： Niu, Junhai;Jian, Heng;Xu, Jianmei;Chen, Changlong;Guo, Quanxin;Liu, Qian;Niu, Junhai;Niu, Junhai;Guo, Yangdong

作者机构：

关键词： Meloidogyne incognita;Rpn7;RNAi;Composite plant;Reproduction;Motility

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN： 0929-1873

年卷期： 2012 年 134 卷 1 期

页码：

收录情况： SCI

摘要： RNA interference (RNAi) techniques provide a major breakthrough in functional analysis for plant parasitic nematodes (PPNs). It offers the possibility of identifying new essential targets and consequently developing new resistance transgenes. To validate the potential of Mi-Rpn7 as a target for controlling root knot nematode Meloidogyne incognita and to evaluate the feasibility of our modified platform for assessing silencing phenotypes, we knocked down the Rpn7 gene of M. incognita using RNAi in vitro and in vivo. After soaking with 408-bp Rpn7 dsRNA, pre-parasitic second-stage juvenile (J2) nematodes showed specific transcript knockdown, resulting in an interrupted locomotion in an attraction assay with Pluronic gel medium, and consequently in a reduction of nematode infection ranging from 55.2% to 66.5%. With in vivo expression of Rpn7 dsRNA in transformed composite plants, the amount of egg mass per gram root tissue was reduced by 34% (P < 0.05) and the number of eggs per gram root tissue was reduced by 50.8% (P < 0.05). Our results demonstrated that the silencing of the Rpn7 gene in M. incognita J2s significantly reduced motility and infectivity. Although it does not confer complete resistance, Mi-Rpn7 RNAi in hairy roots produced significant negative impacts on reproduction and motility of M. incognita. In addition, the presented modified procedure provides technique reference for PPN genes functional analysis or target screening.

分类号：

  • 相关文献

[1]Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita. Zhang, Lei,Chen, Yuyan,Sun, Xiaotang,Huang, Wenkun,Wu, Qingsong,Peng, Deliang,Fan, Chengming,da Silva, Washington.

[2]XocR, a LuxR solo required for virulence in Xanthomonas oryzae pv. oryzicola. Xu, Huiyong,Zhao, Yancun,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan,Xu, Huiyong,Qian, Guoliang,Liu, Fengquan. 2015

[3]Role of the Genes of Type VI Secretion System in Virulence of Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp avenae Strain RS-2. Masum, Md. Mahidul Islam,Yang, Yingzi,Li, Bin,Olaitan, Ogunyemi Solabomi,Chen, Jie,Zhang, Yang,Fang, Yushi,Qiu, Wen,Masum, Md. Mahidul Islam,Wang, Yanli,Sun, Guochang. 2017

[4]Influence of environmental related concentrations of heavy metals on motility parameters and antioxidant responses in sturgeon sperm. Li, Zhi-Hua,Li, Ping,Dzyuba, Borys,Randak, Tomas,Li, Zhi-Hua,Li, Ping,Dzyuba, Borys. 2010

[5]Cryopreservation of sperm from Atlantic halibut (Hippoglossus hippoglossus, L.) for commercial application. Ding, Fuhong,Lei, Jilin,Lall, Santosh P.,Milley, Joyce E.,Li, Jun,Rommens, Melissa. 2011

[6]Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii. Li, Zhi-Hua,Li, Ping,Rodina, Marek,Randak, Tomas,Li, Zhi-Hua,Li, Ping. 2010

[7]Cryopreservation of sperm from turbot (Scophthalmus maximus) and application to large-scale fertilization. Chen, SL,Ji, XS,Yu, GC,Tian, YS,Sha, ZX.

[8]Escherichia coli Type III Secretion System 2 ATPase EivC Is Involved in the Motility and Virulence of Avian Pathogenic Escherichia coli. Wang, Shaohui,Liu, Xin,Xu, Xuan,Yang, Denghui,Wang, Dong,Han, Xiangan,Shi, Yonghong,Tian, Mingxing,Ding, Chan,Yu, Shengqing,Liu, Xin,Peng, Daxin. 2016

[9]Effect of human pharmaceutical Carbamazepine on the quality parameters and oxidative stress in common carp (Cyprinus carpio L.) spermatozoa. Li, Zhi-Hua,Li, Ping,Rodina, Marek,Randak, Tomas,Li, Zhi-Hua,Li, Ping.

[10]Managing Meloidogyne incognita and Bemisia tabaci with thiacloprid in cucumber crops in China. Dong, Sa,Qiao, Kang,Xia, Xiaoming,Wang, Kaiyun,Zhu, Yukun,Wang, Hongyan. 2014

[11]Isolation of nematophagous fungi from eggs and females of Meloidogyne spp. and evaluation of their biological control potential. Aminuzzaman, F. M.,Xie, H. Y.,Duan, W. J.,Sun, B. D.,Liu, X. Z.. 2013

[12]Effectiveness of 1,3-dichloropropene as an alternative to methyl bromide in rotations of tomato (Solanum lycopersicum) and cucumber (Cucumis sativus) in China. Qiao, Kang,Dong, Sa,Xia, Xiaoming,Wang, Kaiyun,Wang, Hongyan,Ji, Xiaoxue. 2012

[13]Effect of abamectin on root-knot nematodes and tomato yield. Qiao, Kang,Liu, Xia,Xia, Xiaoming,Wang, Kaiyun,Wang, Hongyan,Ji, Xiaoxue.

[14]Flame soil disinfestation: A novel, promising, non-chemical method to control soilborne nematodes, fungal and bacterial pathogens in China. Wang, Qiuxia,Yan, Dongdong,Li, Yuan,Ouyang, Canbin,Guo, Meixia,Cao, Aocheng,Mao, Liangang,Wang, Qiuxia,Yan, Dongdong,Li, Yuan,Ouyang, Canbin,Guo, Meixia,Cao, Aocheng.

[15]New Nematotoxic Indoloditerpenoid Produced by Gymnoascus reessii za-130. Liu, Ting,Dong, Dan,Zhang, TaoTao,Liu, Wei-cheng,Meyer, Susan L. F.,Chitwood, David J.,Chauhan, Kamlesh R.,Li, Jun.

[16]GC-MS Analysis of Nematicidal Essential Oil of Mentha canadensis Aerial Parts against Heterodera avenae and Meloidogyne incognita. Ji, Hua,Li, Yun Chao,Wen, Zhi Yu,Li, Hong Tao,Ji, Hua,Li, Yun Chao,Wen, Zhi Yu,Li, Hong Tao,Li, Xiu Hua,Zhang, Hai Xin.

[17]Suppression of Meloidogyne incognita by the endophytic fungus Acremonium implicatum from tomato root galls. Tian, Xueliang,Tian, Xueliang,Yao, Yurong,Chen, Guohua,Mao, Zhenchuan,Xie, Bingyan,Wang, Xiaotian.

[18]Cloning and functional analyses of pepper CaRKNR involved in Meloidogyne incognita resistance. Mao, Zhenchuan,Zhu, Pingping,Liu, Feng,Huang, Yonghong,Ling, Jian,Chen, Guohua,Yang, Yuhong,Feng, Dongxin,Xie, Bingyan.

[19]Gene Cloning and Gene Expression of Hsp90 from Meloidogyne incognita under the Temperature and Heavy Metal Stress. Bai Chunming,Duan Yuxi,Chen Lijie,Wang Yuanyuan,Zhu Xiaofeng,Bai Chunming,Liu Yifei,Zheng Yanan. 2014

[20]Potential use of cucumber (Cucumis sativus L.) endophytic fungi as seed treatment agents against root-knot nematode Meloidogyne incognita. Yan, Xiao-ning,Zheng, Jing-wu,Sikora, Richard A.,Yan, Xiao-ning. 2011

作者其他论文 更多>>

微信小程序