Optimization of Enzymatic Process for Vanillin Extraction Using Response Surface Methodology

文献类型： 外文期刊

第一作者： Gu, Fenglin

作者： Gu, Fenglin;Xu, Fei;Tan, Lehe;Wu, Huasong;Chu, Zhong;Wang, Qinghuang;Gu, Fenglin;Xu, Fei;Tan, Lehe;Wu, Huasong;Chu, Zhong;Wang, Qinghuang;Gu, Fenglin;Xu, Fei;Tan, Lehe;Wu, Huasong;Chu, Zhong;Wang, Qinghuang

作者机构：

关键词： vanillin;extraction;response surface methodology;cellulose;extraction yield

期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）

ISSN： 1420-3049

年卷期： 2012 年 17 卷 8 期

页码：

收录情况： SCI

摘要： Vanillin was extracted from vanilla beans using pretreatment with cellulase to produce enzymatic hydrolysis, and response surface methodology (RSM) was applied to optimize the processing parameters of this extraction. The effects of heating time, enzyme quantity and temperature on enzymatic extraction of vanillin were evaluated. Extraction yield (mg/g) was used as the response value. The results revealed that the increase in heating time and the increase in enzyme quantity (within certain ranges) were associated with an enhancement of extraction yield, and that the optimal conditions for vanillin extraction were: Heating time 6 h, temperature 60 degrees C and enzyme quantity 33.5 mL. Calculated from the final polynomial functions, the optimal response of vanillin extraction yield was 7.62 mg/g. The predicted results for optimal reaction conditions were in good agreement with experimental values.

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