Aggregation and conformational changes of bovine beta-lactoglobulin subjected to dynamic high-pressure microfluidization in relation to antigenicity

文献类型： 外文期刊

第一作者： Zhong, J. Z.

作者： Zhong, J. Z.;Liu, W.;Liu, C. M.;Li, T.;Tu, Z. C.;Luo, S. J.;Cai, X. F.;Xu, Y. J.;Wang, Q. H.

作者机构：

关键词： beta-lactoglobulin;antigenicity;dynamic high-pressure microfluidization (DHPM);protein conformation

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2012 年 95 卷 8 期

页码：

收录情况： SCI

摘要： Our previous research indicated that dynamic high-pressure microfluidization (DHPM) had a significant effect on the antigenicity of beta-lactoglobulin (beta-LG). In this study, aggregation and conformational changes subjected to DHPM (0.1-160 MPa) were investigated in relation to antigenicity. When DHPM pressure increased from 0.1 to 80 MPa, disaggregation of beta-LG samples and partial unfolding of the molecule were accompanied by an increase in beta-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and beta-strands contents, and slight exposure of aromatic amino acid residues. At pressures above 80 MPa, the reaggregation of beta-LG may contribute to the decrease in antigenicity, which was reflected by an increase in particle size, the formation of aggregates, a decrease of in SH and beta-strands contents, and slight changes in aromatic amino acid residues. Aggregation and conformational changes of beta-LG under DHPM was related to its antigenicity.

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