Preparation of a Xanthine Sensor Based on the Immobilization of Xanthine Oxidase on a Chitosan Modified Electrode by Cross-linking

文献类型: 外文期刊

第一作者: Liu Yuge

作者: Liu Yuge;Li Weiming;Wei Changbin;Lu Lingling

作者机构:

关键词: sensor;xanthine oxidase;chitosan;cross-linking

期刊名称:CHINESE JOURNAL OF CHEMISTRY ( 影响因子:6.0; 五年影响因子:3.19 )

ISSN: 1001-604X

年卷期: 2012 年 30 卷 7 期

页码:

收录情况: SCI

摘要: Here in this paper, xanthine oxidase (XOD) was immobilized onto the chitosan (CHT) modified electrode by a simple way of cross-linking with glutaraldehyde (GTD) and 3-aminopropyltriethoxysilane (KH). The electrode displayed a sharp peak to the oxidation of xanthine at a potential about 0.67 V and the optimum of pH for determination was investigated. Under the optimum conditions, the biosensor fabricated on the KH/GTD/XOD/CHT modified electrode showed excellent response to the oxidation of xanthine within the range of 0.5 to 18 mu mol/L with a low detection limit of 0.0215 mu mol/L, a good stability and a high selectivity. The sensor can also be used for the determination of hypoxanthine. The electrochemical results indicated that the immobilized enzyme still retained its biological activity and this provided a new way for the construction of biosensors and determination of xanthine.

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