Characterization of isoproturon biodegradation pathway in Sphingobium sp YBL2
文献类型: 外文期刊
第一作者: Zhang, Ji
作者: Zhang, Ji;Hong, Qing;Li, Chao;Cao, Li;Sun, Ji-Quan;Yan, Xin;Li, Shun-Peng;Li, Qinfen
作者机构:
关键词: Isoproturon;Sphingobium sp YBL2;Metabolites;Biodegradation pathway
期刊名称:INTERNATIONAL BIODETERIORATION & BIODEGRADATION ( 影响因子:4.32; 五年影响因子:4.719 )
ISSN: 0964-8305
年卷期: 2012 年 70 卷
页码:
收录情况: SCI
摘要: In our previous study, a phenylurea herbicides degrading strain Sphingobium sp. YBL2 was isolated. In the present study, the degradation pathway of IPU (Isoproturon) in strain YBL2 was investigated by intermediate metabolites identification. HPLC and/or MS/MS analysis showed that, three intermediate metabolites, MDIPU (1-(4-isopropylphenyl)-3-methylurea), DDIPU (1-(4-isopropylphenyl) urea) and 4-IA (4-lsopropylaniline) was produced by strain YBL2. All of these intermediate metabolites could also be efficiently degraded by strain YBL2. In addition, IPU-induced aniline dioxygenase activity and catechol 2,3-dioxygenase activity were detected in strain YBL2. In combination of all these results, we deduced that the metabolic pathway of IPU in strain YBL2 involves two successive N-demethylations, followed by cleavage of the urea side chain and aromatic ring. (C) 2012 Elsevier Ltd. All rights reserved.
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