A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system

文献类型: 外文期刊

第一作者: Gao, L.

作者: Gao, L.;Shen, W. T.;Yan, P.;Tuo, D. C.;Li, X. Y.;Zhou, P.;Gao, L.;Shen, W. T.;Yan, P.;Tuo, D. C.;Li, X. Y.;Zhou, P.;Gao, L.

作者机构:

关键词: Sos recruitment two-hybrid system;papaya ringspot virus;NIa-Pro;protein-protein interaction

期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )

ISSN: 0001-723X

年卷期: 2012 年 56 卷 1 期

页码:

收录情况: SCI

摘要: The protein-protein interactions between viral and host proteins play an essential role in plant virus infection and host defense. The potyviral nuclear inclusion protein a protease (NIa-Pro) is involved in various steps of viral infection. In this study, the host proteins interacting with papaya ringspot virus (PRSV) NIa-Pro were screened in a Carica papaya L. plant cDNA library using a Sos recruitment two-hybrid system (SRS). We confirmed that the full-length EIF3G, FBPA I, FK506BP, GTPBP, MSRBI, and MTL from papaya can interact specifically with PRSV NIa-Pro in yeast, respectively. These proteins fufill important functions in plant protein translation, biotic and abiotic stress, energy metabolism and signal transduction. In this paper, we discuss possible functions of interactions between these host proteins and NIa-Pro in PRSV infection and their role in host defense.

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