Growth Kinetics of Amyloid-like Fibrils Derived from Individual Subunits of Soy beta-Conglycinin
文献类型: 外文期刊
第一作者: Wang, Jin-Mei
作者: Wang, Jin-Mei;Yang, Xiao-Quan;Yin, Shou-Wei;Xia, Ning;Qi, Jun-Ru;Yuan, De-Bao;Xia, Ning
作者机构:
关键词: beta-conglycinin;individual subunits;polypeptide hydrolysis;conformational changes;assembly;amyloid fibril
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN: 0021-8561
年卷期: 2011 年 59 卷 20 期
页码:
收录情况: SCI
摘要: The amyloid-like fibrillation of soy beta-conglycinin subunits (alpha, alpha', and beta) upon heating (0-20 h) at 85 degrees C and pH 2.0 was characterized using dynamic light scattering, circular didiroism (CD), binding to amyloid dyes (Thioflavin T and Congo red), and atomic force microscopy. The fibrillation of all three subunits was accompanied by progressive polypeptide hydrolysis. The hydrolysis behaviors, fibrillation kinetics, and morphologies of amyloid-like fibrils considerably varied among alpha, alpha', and beta subunits. Faster hydrolysis rates and special fragments were observed for the alpha and alpha' subunits compared to the beta subunit. However, the order of the fibrillation rate and capacity to form beta-sheets was alpha' > beta > alpha, as evidenced by CD and Thioflavin T data. Moreover, sequential growth of twisted screw-structure fibrils, leading to macroscopic fibrils with distinct morphological characteristics, was observed for beta-conglycinin and individual subunits. The different fibrillation kinetics and morphologies of alpha, alpha', and beta subunits appear to be associated with the differences in the amino acid composition and typical sequence of peptides. Besides, the disruption of ordered structure of fibrils occurred upon further heating (6-20 h) due to extensive hydrolysis. These results would suggest that all subunits are involved in the fibrillation of beta-conglycinin, following multiple steps including polypeptide hydrolysis, assembly to amyloid structure, and growth into macroscopic fibrils with a fibril shaving process.
分类号:
- 相关文献
作者其他论文 更多>>
-
Citric Acid Improves Egg White Protein Foaming Characteristics and Meringue 3D Printing Performance
作者:Zhang, Huajiang;Hua, Shihui;Liu, Mengzhuo;Chuang, Rui;Gao, Xin;Li, Hanyu;Xia, Ning;Xiao, Chaogeng
关键词:egg whites; citric acid; foaming property; meringue; rheological property; 3D printing
-
pH shifting treatment of egg yolk granules /salted ovalbumin mixed gels: Reassembled of egg yolk granules effects on heat-induced amorphous aggregates
作者:Wang, Xueying;Meng, Dekun;Wang, Jiaqi;Zhang, Huajiang;Li, Hanyu;Xu, Lina;Xia, Ning;Zhang, Nan;Xiao, Chaogeng;Rayan, Ahmed M.;Ghamry, Mohamed
关键词:Egg yolk granules; pH-shifting; Assembly; Protein unfolding; Protein gel
-
Mechanistic applications of low-temperature plasma in starch-based biopolymer film: A review
作者:Guo, Yanli;Liu, Mengzhuo;Chuang, Rui;Zhang, Huajiang;Li, Hanyu;Xu, Lina;Xia, Ning;Xiao, Chaogeng;Rayan, Ahmed M.;Ghamry, Mohamed
关键词:Low-temperature plasma; Starch films; Oxidation; Cross-linking; Coordination reaction
-
Extraction of Abandoned Cropland Using Multisource Remote Sensing Images in Suburban Regions: A Case Study of Zengcheng, Guangdong Province
作者:Feng, Shanshan;Jiang, Shun;Liu, Xu;Zhang, Lei;Gan, Yangying;Zhou, Canfang;Feng, Shanshan;Jiang, Shun;Liu, Xu;Zhang, Lei;Gan, Yangying;Xia, Ning;Zhou, Canfang;Xia, Ning;Wu, Wenbin;Wu, Wenbin
关键词:Spatial resolution; Accuracy; Remote sensing; Data mining; Normalized difference vegetation index; Meters; Urban areas; Crops; Graphical models; Distribution functions; Abandoned cropland; land use classification; normalized difference vegetation index (NDVI) maximum value; unplanted cropland; Zengcheng
-
Exploring the potential of egg yolk spheres to stabilize foams by pH shift: Structural, interfacial, and capillary foam effects
作者:Hou, Jingjie;Jiang, Zhongtao;Liu, Mengzhou;Zhang, Huajiang;Li, Hanyu;Xia, Ning;Xu, Lina;Zhang, Lili;Li, Hanyu;Zhang, Lili;Xiao, Chaogeng
关键词:Egg yolk sphere; pH shift; Foaming properties; Protein structure; Interfacial dynamics; Capillary foam
-
Bioactives derived from egg by-products: Preparation, health benefits, and high-value-added applications
作者:Huang, Yue;Chuang, Rui;Liu, Mengzhuo;Zhang, Huajiang;Li, Hanyu;Xu, Lina;Xia, Ning;Xiao, Chaogeng;Rayan, Ahmed M.;Ghamry, Mohamed
关键词:Egg by-products; Bioactives; Health benefits; Valorization; Sustainable development
-
Biocompatible porous material templated from gliadin particle and gellan gum co-stabilized wet foam
作者:Zhou, Fu-Zhen;Zhang, Long -Tao;Zhou, Fu-Zhen;Wei, Shu-Heng;Yang, Xiao-Quan;Yin, Shou-Wei;Yang, Tao;Xiang, Huan;Zhu, Jian-Hua;Zhu, Jian-Hua
关键词:Pickering stabilization; Gliadin particles; Gellan gum; Foam; Porous