Plant regeneration system from cotyledons-derived calluses cultures of Stylosanthes guianensis cv. 'Reyan 2'

文献类型: 外文期刊

第一作者: Yuan, Xuejun

作者: Yuan, Xuejun;Liu, Guodao;Yuan, Xuejun;Wang, Zhiyong;Liao, Li;Wang, Zhiyong

作者机构:

关键词: Stylosanthes guianensis Sw. cv. Reyan 2;callus;cotyledons;plant regeneration

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2011 年 10 卷 56 期

页码:

收录情况: SCI

摘要: The objective of this study was to successfully establish plant regeneration system with cotyledons of Stylosanthes guianensis Sw. cv. 'Reyan 2' as explants. In this study, the following results were obtained; (1) the highest rates of callus induction on medium MS with 3.0 mg L(-1) 2, 4-D with cotyledons as explants were 74%. The above medium of formulation was adopted for subculture; (2) the highest rates of callus differentiation medium MS with 3.0 mg L(-1) 6-BA with cotyledons as explants was 66%; (3) the optimum medium of shoot growth was MS medium plus 1.0 mg L(-1) 6-BA, 0.5 mg L(-1) NAA and 0.4 mg L(-1) GA; (4) the rooting rate was 60% on optimum rooting medium with 0.2 mg L(-1) NAA; (5) the survival rate of plant transplanting was 70% after transferred to pots with garden soil. In conclusion, the efficient plant regeneration system developed here will be helpful for rapid micropropagation and further genetic improvement in S. guianensis Sw. cv. 'Reyan 2'.

分类号:

  • 相关文献

[1]A rapid shoot regeneration protocol from the cotyledons of hemp (Cannabis sativa L.). Cheng Chaohua,Zang Gonggu,Zhao Lining,Gao Chunsheng,Tang Qing,Chen Jianhua,Su Jianguang,Guo Xinbo,Cheng Chaohua,Peng Dingxiang.

[2]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[3]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[4]Direct and indirect shoot and bulblet regeneration from cultured leaf explants of Lilium pumilum, an endangered species. Jin, Shumei,Wang, Ji,Sun, Dan,Liu, Shengkui,Wang, Xinwang,Genovesi, A. D.,Li, Guoliang. 2014

[5]Effect of mouse calcineurin on induction and growth of rice callus transformed by the calcineurin gene. Ma, Xujun,Qian, Qian,Yang, Chuanping,Liu, Guifeng,Zhu, Dahai. 2006

[6]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[7]Plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis. Zhou, Quan-Nan,Huang, Tian-Dai,Li, Wei-Guo,Sun, Ai-Hua,Dai, Xue-Mei,Li, Zhe,Jiang, Ze-Hai. 2010

[8]Effect of brassinolide on callus growth and regeneration in Spartina patens (Poaceae). Lu, Z,Huang, M,Ge, DP,Yang, YH,Cao, XN,Qin, P,She, JM. 2003

[9]Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis. Tan, Deguan,Sun, Xuepiao,Zhang, Jiaming. 2011

[10]Establishment of Adventitious Bud Regeneration System from Bulb of Muscari Botryoides Mill. Sun, Hongmei,Zhang, Jiao,Song, Lina,Sun, Hongmei,Tao, Chengguang. 2011

[11]Production of Green Fluorescent Protein in Transgenic Rice Seeds. Gao, Jing,Shen, Chunxiu,Fang, Zhen,Xia, Yumei,Cao, Mengliang,Yuan, Longping,Cao, Mengliang,Cao, Mengliang.

[12]Cell types in the wild type callus of rice (Oryza sativa L.) as revealed by screening for salt tolerant lines with selection pressure. Liua, P,Baob, W. 1998

[13]Somatic embryogenesis from thin epidermal layers in sunflower and chromosomal regions controlling the response. Huang, XianQun,Nabipour, Alireza,Gentzbittel, Laurent,Sarrafi, Ahmad.

[14]Plant regeneration via somatic embryogenesis in cotton. Bao-Hong Zhang,Fang Liu,Chang-Bing Yao. 2000

[15]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[16]Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos. Zhang Wei,Wang Xin-min,Yin Gui-xiang,Wang Ke,Du Li-pu,Xiao Le-le,Ye Xing-guo,Fan Rong. 2015

[17]Plant regeneration from cotyledon of Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How). He, Xiaoming,Xie, Dasen,Peng, Qingwu,Mu, Lixia. 2007

[18]Plant regeneration from cell suspension-derived protoplasts of Populus x beijingensis. Cai, Xiao,Kang, Xiang-Yang,Cai, Xiao. 2014

[19]Direct somatic embryogenesis from leaf and petiole explants of Spathiphyllum 'Supreme' and analysis of regenerants using flow cytometry. Zhao, Jietang,Henny, Richard J.,Chen, Jianjun,Cui, Jin,Liu, Juanxu,Liao, Feixiong,Chen, Jianjun. 2012

[20]Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos. Wu, Shen-Jie,Wang, Hai-Hai,Li, Fei-Fei,Chen, Tian-Zi,Zhang, Jie,Jiang, Yan-Jie,Ding, Yezhang,Guo, Wang-Zhen,Zhang, Tian-Zhen,Wu, Shen-Jie.

作者其他论文 更多>>