A SIMPLE AND EFFICIENT METHOD FOR ISOLATION OF PINEAPPLE PROTOPLASTS

文献类型： 外文期刊

第一作者： Zhao, Weifeng

作者： Zhao, Weifeng;Wei, Changbin;Sun, Guangming;Zhao, Weifeng;Yang, Wenxiu

作者机构：

关键词： pineapple;protoplast;isolation;enzyme

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2011 年 25 卷 3 期

页码：

收录情况： SCI

摘要： Protoplasts have showed great value for new germplasms generation. A method was described for the isolation of large numbers of pineapple protoplasts. The procedure utilized 5% mannitol solution as the primary osmoticum and an enzyme mixture of 1.5% macerozyme R-10, 1.5% cellulase R-10 and 5% mannitol solution, pH 5.5, followed by differential and gradient centrifugations. Overall, the method was found to be a simplified and effective alternative to those previously described for pineapple protoplast isolation, obtaining the highest protoplast yield (3.6 x 10(6)/g FW) and the highest protoplast viability (88.9%). Biotechnol. & Biotechnol. Eq. 2011, 25(3), 2464-2467

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