Factors that influence the yield and viability of protoplasts from Carica papaya L.
文献类型: 外文期刊
第一作者: Zhang, Jianbo
作者: Zhang, Jianbo;Shen, Wentao;Yan, Pu;Li, Xiaoying;Zhou, Peng
作者机构:
关键词: Carica papaya L.;isolation conditions;protoplasts;Taguchi experimental design;viability;yields
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 26 期
页码:
收录情况: SCI
摘要: The effects of four main factors (the concentration of cellulase R-10, macerozyme R-10 and D-mannitol, and duration of enzyme incubation) determining yield and viability of protoplasts isolation were investigated using Taguchi experimental design to optimize protoplasts isolation conditions from the leaves of Carica papaya L. seedlings. The results showed that the concentration of cellulase R-10 and incubation time were key factors affecting the protoplast yield; D-mannitol concentration played a significant role in protoplast viability. Moreover, the result of single factor tests revealed that pH of the enzyme solution significantly influenced both yield and viability of protoplasts. The highest yield of more than 1.5 x 10(7) protoplasts g(-1) fresh weight (FW) with more than 90% viability were consistently obtained by the optimum isolation conditions: enzyme combination of 1.2% cellulase R-10, 0.3% macerozyme R-10 and 0.44 M D-mannitol, pH 5.8 and incubation for 13 h at 26 degrees C with a shaker speed of 60 rpm in darkness. This study will facilitate to obtain sufficient yields and viability of protoplasts for subsequent novel applications in papaya biotechnology such as the gene functional analysis and mechanism of PRSV infection in papaya protoplasts.
分类号:
- 相关文献
作者其他论文 更多>>
-
A telosma mosaic virus-based vector for foreign gene expression and virus-induced gene silencing in passion fruit
作者:Wang, Xiaoqing;Qin, Li;Cui, Hongguang;Dai, Zhaoji;Shen, Wentao;Shen, Wentao;Qiu, Wenping
关键词:
-
A Comprehensive Screening of the Interactors of Areca Palm Necrotic Ringspot Virus (ANRSV) HCPro2 Highlights the Proviral Roles of eIF4A and PGK in Viral Infection
作者:Qin, Li;Liu, Peilan;Dai, Zhaoji;Cui, Hongguang;Shen, Wentao
关键词:
Potyviridae ; leader protease; cysteine protease; eukaryotic initiation factor 4A; phosphoglycerate kinase -
Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells
作者:Wu, Jiawei;Zhang, Jianbo;Shu, Wanyu;Cao, Huijuan;Jiang, Chunhua;Wang, Sitong;Wu, Fanwu;Wu, Chenxi;Feng, Wei;Meng, Ran;Wang, Xiuping;Kong, Lingyu;Wu, Chenxi
关键词:Taraxacum sinicum Kitag; root extract; tumor invasion; Notch; Drosophila; breast cancer
-
Genetic Tendency Analysis and Comprehensive Antioxidant Activity Evaluation of Leaves and Flowers of Loquat F1 Generation
作者:Zhu, Qixuan;Li, Xiaoying;Ge, Hang;Wang, Zhixuan;Wang, Binjun;Chen, Junwei;Xu, Hongxia;Zhu, Qixuan
关键词:loquat; total phenolics; flavonoids; antioxidant activity; correlation
-
Genome-wide identification of UDP-glycosyltransferases involved in flavonol glycosylation induced by UV-B irradiation in Eriobotrya japonica
作者:Qian, Jiafei;Ren, Chuanhong;Wang, Fan;Cao, Yunlin;Guo, Yan;Zhao, Xiaoyong;Liu, Yilong;Zhu, Changqing;Chen, Kunsong;Li, Xian;Qian, Jiafei;Ren, Chuanhong;Wang, Fan;Cao, Yunlin;Guo, Yan;Zhao, Xiaoyong;Liu, Yilong;Zhu, Changqing;Chen, Kunsong;Li, Xian;Li, Xiaoying;Xu, Hongxia;Chen, Junwei;Ren, Chuanhong
关键词:Loquat; Flavonol glycoside; UGT; Galactosyltransferase; Glucosyltransferase; Rhamnosyltransferase; UV-B
-
Physiological and biochemical responses of pepper (Capsicum annuum) to cadmium stress: The mitigating effects of exogenous abscisic acid
作者:Tu, Dehui;Tian, Xiao;Liang, Chuangjing;Zhou, Peng;Wang, Yongping;Xing, Dan;Liu, Yizhang
关键词:Cell wall; Abscisic acid; Oxidative stress; Cd stress; Subcellular distribution
-
Genome-wide identification, tissue expression pattern, and salt stress response analysis of the NAC gene family in Thinopyrum elongatum
作者:Sun, Jilin;Zhang, Jiaqi;Meng, Fansen;Li, Jiangong;Sun, Silong;Cui, Xiaokuo;Wu, Changai;Zhou, Shenghui;Zhou, Peng;Han, Long
关键词:
TeNACs ;Th. elongatum ; Expression pattern; Salt stress