Molecular cloning and immune characterization of CIITA in artificially challenged Chinese tongue sole (Cynoglossus semilaevis) with Vibrio harveyi

文献类型： 外文期刊

第一作者： Chen, Zhangfan

作者： Chen, Zhangfan;Wang, Lei;Xu, Xiwen;Zhou, Qian;Wang, Jie;Chen, Yadong;Wang, Na;Gong, Zhihong;Chen, Songlin;Chen, Zhangfan;Wang, Lei;Xu, Xiwen;Zhou, Qian;Chen, Yadong;Wang, Na;Chen, Songlin;Chen, Zhangfan;Wang, Lei;Xu, Xiwen;Zhou, Qian;Chen, Yadong;Wang, Na;Gong, Zhihong;Chen, Songlin;Wang, Jie

作者机构：

关键词： CIITA; Cynoglossus semilaevis; MHC II; Immunity; Vibrio harveyi

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN： 0145-305X

年卷期： 2021 年 121 卷

页码：

收录情况： SCI

摘要： In mammals, Class II, major histocompatibility complex (MHC II) transactivator (CIITA) recognizes microbial pathogens and triggers immune responses. In Chinese tongue sole Cynoglossus semilaevis, Cs-CIITA was prevalently expressed in various tissues. Cs-CIITA, Cs-MHC IIA and Cs-MHC IIB were expressed significantly higher in skin in susceptible families infected with Vibrio harveyi, while higher expression of Cs-CIITA and Cs-MHC IIB was examined in liver in resistant families. In addition, the three genes were up-regulated in gill, skin, intestine, liver, spleen and kidney at 48 h or 72 h after V. harveyi infection. Furthermore, the three genes were co-expressed in the epithelial mucous cells of gill, skin, and intestine. Knockdown of Cs-CIITA regulates the expression of other inflammation-related genes, including CD40, IL-1 beta, IL-8, RelB, NF kappa B, and Myd88. These results suggest that CIITA functions in the inflammatory responses of C. semilaevis against V. harveyi, via MHC II transcriptional regulation.

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