Lipoamide or lipoic acid stimulates mitochondrial biogenesis in 3T3-L1 adipocytes via the endothelial NO synthase-cGMP-protein kinase G signalling pathway

文献类型： 外文期刊

第一作者： Liu, Jiankang

作者： Liu, Jiankang;Shen, Weili;Shen, Weili;Hao, Jiejie;Feng, Zhihui;Tian, Chuan;Feng, Zhihui;Tian, Chuan;Chen, Weijun;Packer, Lester;Shi, Xianglin;Zang, Weijin

作者机构：

关键词： lipoic acid;lipoamide;mitochondria;peroxisome proliferator-activated receptor-gamma co-activator-1 alpha (PGC-1 alpha);mitochondrial transcription factor A (TFAM);nuclear respiratory factor 1 (NRF1)

期刊名称：BRITISH JOURNAL OF PHARMACOLOGY （ 影响因子：8.739； 五年影响因子：7.954 ）

ISSN： 0007-1188

年卷期： 2011 年 162 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUND AND PURPOSE Metabolic dysfunction due to loss of mitochondria plays an important role in diabetes, and stimulation of mitochondrial biogenesis by anti-diabetic drugs improves mitochondrial function. In a search for potent stimulators of mitochondrial biogenesis, we examined the effects and mechanisms of lipoamide and alpha-lipoic acid (LA) in adipocytes. EXPERIMENTAL APPROACH Differentiated 3T3-L1 adipocytes were treated with lipoamide or LA. Mitochondrial biogenesis and possible signalling pathways were examined. KEY RESULTS Exposure of 3T3-L1 cells to lipoamide or LA for 24 h increased the number and mitochondrial mass per cell. Such treatment also increased mitochondrial DNA copy number, protein levels and expression of transcription factors involved in mitochondrial biogenesis, including PGC-1 alpha, mitochondrial transcription factor A and nuclear respiratory factor 1. Lipoamide produced these effects at concentrations of 1 and 10 mu mol center dot L-1, whereas LA was most effective at 100 mu mol center dot L-1. At 10 mu mol center dot L-1, lipoamide, but not LA, stimulated mRNA expressions of PPAR-gamma, PPAR-alpha and CPT-1 alpha. The potency of lipoamide was 10-100-fold greater than that of LA. Lipoamide dose-dependently stimulated expression of endothelial nitric oxide synthase (eNOS) and formation of cGMP. Knockdown of eNOS (with small interfering RNA) prevented lipoamide-induced mitochondrial biogenesis, which was also blocked by the soluble guanylate cyclase inhibitor, ODQ and the protein kinase G (PKG) inhibitor, KT5823. Thus, stimulation of mitochondrial biogenesis by lipoamide involved signalling via the eNOS-cGMP-PKG pathway. CONCLUSIONS AND IMPLICATIONS Our data suggest that lipoamide is a potent stimulator of mitochondrial biogenesis in adipocyte, and may have potential therapeutic application in obesity and diabetes.

分类号：

  • 相关文献

[1]A phosphopantetheinyl transferase that is essential for mitochondrial fatty acid biosynthesis. Guan, Xin,Nikolau, Basil J.,Guan, Xin,Abramson, Alex,Nikolau, Basil J.,Chen, Hui,Man, Huimin,Wu, Jinxia,Yu, Oliver,Chen, Hui,Man, Huimin,Abramson, Alex,Wu, Jinxia,Yu, Oliver,Nikolau, Basil J..

[2]Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells. Cai, Xuan,Chen, Xiaolian,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Cai, Xuan,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Chen, Xiaolian. 2013

[3]Sulfide-based ATP production in Urechis unicinctus. Ma Zhuojun,Bao Zhenmin,Wang Sifeng,Zhang Zhifeng,Ma Zhuojun,Wang Sifeng. 2010

[4]Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed. Xin, Xia,Tian, Qian,Yin, Guangkun,Chen, Xiaoling,Zhang, Jinmei,Lu, Xinxiong,Tian, Qian,Ng, Sophia,Ng, Sophia. 2014

[5]Mechanism of Free Zn(2+) Enhancing Inhibitory Effects of EGCG on the Growth of PC-3 Cells: Interactions with Mitochondria. Shen, Shengrong,Yu, Haining,Sun, Shili,Das, Undurti N.. 2009

[6]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[7]Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties. Huang, Wenjing,Oo, Thet Lwin,Gu, Minghua,Zhan, Jie,Wang, Aiqin,He, Long-Fei,He, Huyi,He, Long-Fei. 2018

[8]Transport of Calcium Ions into Mitochondria. Xu, Zhaolong,Zhang, Dayong,He, Xiaolan,Huang, Yihong,Shao, Hongbo,Shao, Hongbo. 2016

[9]The E-Subgroup Pentatricopeptide Repeat Protein Family in Arabidopsis thaliana and Confirmation of the Responsiveness PPR96 to Abiotic Stresses. Liu, Jia-Ming,Zhao, Juan-Ying,Guo, Chang-Hong,Liu, Jia-Ming,Zhao, Juan-Ying,Lu, Pan-Pan,Chen, Ming,Xu, Zhao-Shi,Ma, You-Zhi. 2016

[10]iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Xu, Wentao,Luo, Yunbo,Huang, Kunlun,Wang, Yan,Wang, Yan,Peng, Xiaoli,Yang, Zhuojun,Zhao, Weiwei,Xu, Wentao,Hao, Junran,Wu, Weihong,Shen, Xiao Li,Luo, Yunbo,Huang, Kunlun,Peng, Xiaoli,Shen, Xiao Li. 2017

[11]Protective effect of Momordica charantia water extract against liver injury in restraint-stressed mice and the underlying mechanism. Deng, Yuanyuan,Tang, Qin,Zhang, Yan,Zhang, Ruifen,Wei, Zhencheng,Tang, Xiaojun,Zhang, Mingwei. 2017

[12]Anemone rivularis inhibits pyruvate dehydrogenase kinase activity and tumor growth. Chung, Tae-Wook,Lee, Jung Hee,Choi, Hee-Jung,Park, Mi-Ju,Kim, Eun-Yeong,Han, Jung Ho,Ha, Ki-Tae,Chung, Tae-Wook,Lee, Jung Hee,Choi, Hee-Jung,Park, Mi-Ju,Kim, Eun-Yeong,Han, Jung Ho,Ha, Ki-Tae,Jang, Se Bok,Lee, Syng-Ook,Lee, Sang Woo,Hang, Jin,Yi, Li Wan.

[13]Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA. Meng, Xian Hong,Wang, Qing Yin,Liu, Ping,Kong, Jie,Meng, Xian Hong,Jang, In Kwon.

[14]Mitochondrial proteins differential expression during honeybee (Apis mellifera L.) queen and worker larvae caste determination. Begna, Desalegn,Fang, Yu,Feng, Mao,Li, Jianke.

[15]Separation of motile sperm for in vitro fertilization from frozen-thawed bull semen using progesterone induction on a microchip. Li, Jingchun,Ning, Bolin,Luo, Yinghua,Guo, Li,Wei, Guosheng,Liu, Shengjun,Zhang, Ying,Zhang, Aizhong,Wu, Rui,Li, Yanbing,Cao, Xinyan.

[16]Sulfated lentinan induced mitochondrial dysfunction leads to programmed cell death of tobacco BY-2 cells. Wang, Jie,Shen, Lili,Qian, Yumei,Yang, Jinguang,Wang, Fenglong,Wang, Yaofeng.

[17]Aluminum chloride caused liver dysfunction and mitochondrial energy metabolism disorder in rat. Xu, Feibo,Liu, Yanfen,Yu, Kaiyuan,Song, Miao,Li, Yanfei,Liu, Yanfen,Zhu, Yanzhu,Zhao, Hansong.

[18]Effects of powdery mildew infection on chloroplast and mitochondrial functions in rubber tree. Wang, Li-Feng,Wang, Meng,Zhang, Yu.

[19]Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes. Zhao, Xue-Ming,Hao, Hai-Sheng,Du, Wei-Hua,Zhao, Shan-Jiang,Wang, Hao-Yu,Wang, Na,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2016

[20]Gene duplication and transfer events in plant mitochondria genome. Xiong, Ai-Sheng,Peng, Ri-He,Zhuang, Jing,Gao, Feng,Zhu, Bo,Fu, Xiao-Yan,Xue, Yong,Jin, Xiao-Fen,Tian, Yong-Sheng,Zhao, Wei,Yao, Quan-Hong. 2008

作者其他论文 更多>>

微信小程序